TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.
dc.contributor.author | Lindy, Amanda S | |
dc.contributor.author | Parekh, Puja K | |
dc.contributor.author | Zhu, Richard | |
dc.contributor.author | Kanju, Patrick | |
dc.contributor.author | Chintapalli, Sree V | |
dc.contributor.author | Tsvilovskyy, Volodymyr | |
dc.contributor.author | Patterson, Randen L | |
dc.contributor.author | Anishkin, Andriy | |
dc.contributor.author | van Rossum, Damian B | |
dc.contributor.author | Liedtke, Wolfgang B | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2016-03-01T14:18:23Z | |
dc.date.issued | 2014-09-02 | |
dc.description.abstract | Animals need to sense and react to potentially dangerous environments. TRP ion channels participate in nociception, presumably via Ca(2+) influx, in most animal species. However, the relationship between ion permeation and animals' nocifensive behaviour is unknown. Here we use an invertebrate animal model with relevance for mammalian pain. We analyse the putative selectivity filter of OSM-9, a TRPV channel, in osmotic avoidance behaviour of Caenorhabditis elegans. Using mutagenized OSM-9 expressed in the head nociceptor neuron, ASH, we study nocifensive behaviour and Ca(2+) influx. Within the selectivity filter, M(601)-F(609), Y604G strongly reduces avoidance behaviour and eliminates Ca(2+) transients. Y604F also abolishes Ca(2+) transients in ASH, while sustaining avoidance behaviour, yet it disrupts behavioral plasticity. Homology modelling of the OSM-9 pore suggests that Y(604) may assume a scaffolding role. Thus, aromatic residues in the OSM-9 selectivity filter are critical for pain behaviour and ion permeation. These findings have relevance for understanding evolutionary roots of mammalian nociception. | |
dc.identifier | ||
dc.identifier | ncomms5734 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | Springer Science and Business Media LLC | |
dc.relation.ispartof | Nat Commun | |
dc.relation.isversionof | 10.1038/ncomms5734 | |
dc.subject | Amino Acid Sequence | |
dc.subject | Amino Acid Substitution | |
dc.subject | Animals | |
dc.subject | Avoidance Learning | |
dc.subject | Caenorhabditis elegans | |
dc.subject | Caenorhabditis elegans Proteins | |
dc.subject | Calcium | |
dc.subject | Calcium Signaling | |
dc.subject | Gene Expression | |
dc.subject | Ion Transport | |
dc.subject | Models, Molecular | |
dc.subject | Molecular Sequence Data | |
dc.subject | Mutation | |
dc.subject | Nerve Tissue Proteins | |
dc.subject | Nociception | |
dc.subject | Nociceptors | |
dc.subject | Recombinant Fusion Proteins | |
dc.subject | Sequence Alignment | |
dc.subject | Structural Homology, Protein | |
dc.subject | TRPV Cation Channels | |
dc.title | TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour. | |
dc.type | Journal article | |
duke.contributor.orcid | Liedtke, Wolfgang B|0000-0003-4166-5394 | |
pubs.author-url | ||
pubs.begin-page | 4734 | |
pubs.organisational-group | Anesthesiology | |
pubs.organisational-group | Basic Science Departments | |
pubs.organisational-group | Clinical Science Departments | |
pubs.organisational-group | Duke | |
pubs.organisational-group | Duke Institute for Brain Sciences | |
pubs.organisational-group | Institutes and Provost's Academic Units | |
pubs.organisational-group | Neurobiology | |
pubs.organisational-group | Neurology | |
pubs.organisational-group | Neurology, Headache and Pain | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | University Institutes and Centers | |
pubs.publication-status | Published online | |
pubs.volume | 5 |
Files
Original bundle
- Name:
- TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.pdf
- Size:
- 2.33 MB
- Format:
- Adobe Portable Document Format