Swimming Exercise and Transient Food Deprivation in Caenorhabditis elegans Promote Mitochondrial Maintenance and Protect Against Chemical-Induced Mitotoxicity.

dc.contributor.author

Hartman, Jessica H

dc.contributor.author

Smith, Latasha L

dc.contributor.author

Gordon, Kacy L

dc.contributor.author

Laranjeiro, Ricardo

dc.contributor.author

Driscoll, Monica

dc.contributor.author

Sherwood, David R

dc.contributor.author

Meyer, Joel N

dc.date.accessioned

2018-06-01T13:14:20Z

dc.date.available

2018-06-01T13:14:20Z

dc.date.issued

2018-05-29

dc.date.updated

2018-06-01T13:14:19Z

dc.description.abstract

Exercise and caloric restriction improve health, including reducing risk of cardiovascular disease, neurological disease, and cancer. However, molecular mechanisms underlying these protections are poorly understood, partly due to the cost and time investment of mammalian long-term diet and exercise intervention studies. We subjected Caenorhabditis elegans nematodes to a 6-day, twice daily swimming exercise regimen, during which time the animals also experienced brief, transient food deprivation. Accordingly, we included a non-exercise group with the same transient food deprivation, a non-exercise control with ad libitum access to food, and a group that exercised in food-containing medium. Following these regimens, we assessed mitochondrial health and sensitivity to mitochondrial toxicants. Exercise protected against age-related decline in mitochondrial morphology in body-wall muscle. Food deprivation increased organismal basal respiration; however, exercise was the sole intervention that increased spare respiratory capacity and proton leak. We observed increased lifespan in exercised animals compared to both control and transiently food-deprived nematodes. Finally, exercised animals (and to a lesser extent, transiently food-deprived animals) were markedly protected against lethality from acute exposures to the mitotoxicants rotenone and arsenic. Thus, swimming exercise and brief food deprivation provide effective intervention in C. elegans, protecting from age-associated mitochondrial decline and providing resistance to mitotoxicant exposures.

dc.identifier.issn

2045-2322

dc.identifier.issn

2045-2322

dc.identifier.uri

https://hdl.handle.net/10161/17077

dc.language

eng

dc.publisher

Springer Science and Business Media LLC

dc.relation.ispartof

Scientific reports

dc.relation.isversionof

10.1038/s41598-018-26552-9

dc.title

Swimming Exercise and Transient Food Deprivation in Caenorhabditis elegans Promote Mitochondrial Maintenance and Protect Against Chemical-Induced Mitotoxicity.

dc.type

Journal article

duke.contributor.orcid

Sherwood, David R|0000-0002-4448-6917

duke.contributor.orcid

Meyer, Joel N|0000-0003-1219-0983

pubs.issue

1

pubs.organisational-group

Nicholas School of the Environment

pubs.organisational-group

Duke

pubs.organisational-group

Civil and Environmental Engineering

pubs.organisational-group

Pratt School of Engineering

pubs.organisational-group

Environmental Sciences and Policy

pubs.organisational-group

Duke Cancer Institute

pubs.organisational-group

Institutes and Centers

pubs.organisational-group

School of Medicine

pubs.organisational-group

Duke Global Health Institute

pubs.organisational-group

University Institutes and Centers

pubs.organisational-group

Institutes and Provost's Academic Units

pubs.organisational-group

Staff

pubs.publication-status

Published

pubs.volume

8

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Hartman et al., 2018.pdf
Size:
4.25 MB
Format:
Adobe Portable Document Format