Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants.

Prichard, Heather L

Schroeder, Thies

Reichert, William M

Klitzman, Bruce

United States

2015-07-30T22:37:11Z

2010-09-01

BACKGROUND: The bioluminescence technique was used to quantify the local glucose concentration in the tissue surrounding subcutaneously implanted polyurethane material and surrounding glucose sensors. In addition, some implants were coated with a single layer of adipose-derived stromal cells (ASCs) because these cells improve the wound-healing response around biomaterials. METHODS: Control and ASC-coated implants were implanted subcutaneously in rats for 1 or 8 weeks (polyurethane) or for 1 week only (glucose sensors). Tissue biopsies adjacent to the implant were immediately frozen at the time of explant. Cryosections were assayed for glucose concentration profile using the bioluminescence technique. RESULTS: For the polyurethane samples, no significant differences in glucose concentration within 100 μm of the implant surface were found between bare and ASC-coated implants at 1 or 8 weeks. A glucose concentration gradient was demonstrated around the glucose sensors. For all sensors, the minimum glucose concentration of approximately 4 mM was found at the implant surface and increased with distance from the sensor surface until the glucose concentration peaked at approximately 7 mM at 100 μm. Then the glucose concentration decreased to 5.5-6.5 mM more than 100 μmm from the surface. CONCLUSIONS: The ASC attachment to polyurethane and to glucose sensors did not change the glucose profiles in the tissue surrounding the implants. Although most glucose sensors incorporate a diffusion barrier to reduce the gradient of glucose and oxygen in the tissue, it is typically assumed that there is no steep glucose gradient around the sensors. However, a glucose gradient was observed around the sensors. A more complete understanding of glucose transport and concentration gradients around sensors is critical.

http://www.ncbi.nlm.nih.gov/pubmed/20920425

1932-2968

https://hdl.handle.net/10161/10337

eng

SAGE Publications

J Diabetes Sci Technol

10.1177/193229681000400504

Animals

Biological Transport

Biopsy

Biosensing Techniques

Glucose

Implants, Experimental

Luminescence

Male

Models, Animal

Oxygen

Polyurethanes

Rats

Rats, Inbred Lew

Skin

Stromal Cells

Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants.

Journal article

http://www.ncbi.nlm.nih.gov/pubmed/20920425

1055

1062

5

Basic Science Departments

Biomedical Engineering

Cell Biology

Clinical Science Departments

Duke

Faculty

Pratt School of Engineering

School of Medicine

Surgery

Surgery, Plastic, Maxillofacial, and Oral Surgery

Published online

4