Variation in frequency of plastid RNA editing within Adiantum implies rapid evolution in fern plastomes.

Fauskee, Blake D; Sigel, Erin M; Pryer, Kathleen M; Grusz, Amanda L

Variation in frequency of plastid RNA editing within Adiantum implies rapid evolution in fern plastomes.

dc.contributor.author	Fauskee, Blake D
dc.contributor.author	Sigel, Erin M
dc.contributor.author	Pryer, Kathleen M
dc.contributor.author	Grusz, Amanda L
dc.date.accessioned	2021-11-09T16:33:19Z
dc.date.available	2021-11-09T16:33:19Z
dc.date.issued	2021-05-09
dc.date.updated	2021-11-09T16:33:18Z
dc.description.abstract	Premise Recent studies of plant RNA editing have demonstrated that the number of editing sites can vary widely among large taxonomic groups (orders, families). Yet, very little is known about intrageneric variation in frequency of plant RNA editing, and no study has been conducted in ferns. Methods We determined plastid RNA-editing counts for two species of Adiantum (Pteridaceae), A. shastense and A. aleuticum, by implementing a pipeline that integrated read-mapping and SNP-calling software to identify RNA-editing sites. We then compared the edits found in A. aleuticum and A. shastense with previously published edits from A. capillus-veneris by generating alignments for each plastid gene. Results We found direct evidence for 505 plastid RNA-editing sites in A. aleuticum and 509 in A. shastense, compared with 350 sites in A. capillus-veneris. We observed striking variation in the number and location of the RNA-editing sites among the three species, with reverse (U-to-C) editing sites showing a higher degree of conservation than forward (C-to-U) sites. Additionally, sites involving start and stop codons were highly conserved. Conclusions Variation in the frequency of RNA editing within Adiantum implies that RNA-editing sites can be rapidly gained or lost throughout evolution. However, varying degrees of conservation between both C-to-U and U-to-C sites and sites in start or stop codons, versus other codons, hints at the likely independent origin of both types of edits and a potential selective advantage conferred by RNA editing.
dc.identifier.issn	0002-9122
dc.identifier.issn	1537-2197
dc.identifier.uri	https://hdl.handle.net/10161/23975
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	American journal of botany
dc.relation.isversionof	10.1002/ajb2.1649
dc.subject	Plastids
dc.subject	Ferns
dc.subject	Adiantum
dc.subject	Phylogeny
dc.subject	RNA Editing
dc.title	Variation in frequency of plastid RNA editing within Adiantum implies rapid evolution in fern plastomes.
dc.type	Journal article
duke.contributor.orcid	Pryer, Kathleen M\|0000-0002-9776-6736
pubs.begin-page	820
pubs.end-page	827
pubs.issue	5
pubs.organisational-group	Trinity College of Arts & Sciences
pubs.organisational-group	Biology
pubs.organisational-group	Duke Science & Society
pubs.organisational-group	Duke
pubs.organisational-group	Initiatives
pubs.organisational-group	Institutes and Provost's Academic Units
pubs.publication-status	Published
pubs.volume	108