A blinded randomized assessment of laser Doppler flowmetry efficacy in standardizing outcome from intraluminal filament MCAO in the rat.
dc.contributor.author | Taninishi, Hideki | |
dc.contributor.author | Jung, Jin Yong | |
dc.contributor.author | Izutsu, Miwa | |
dc.contributor.author | Wang, Zhengfeng | |
dc.contributor.author | Sheng, Huaxin | |
dc.contributor.author | Warner, David S | |
dc.date.accessioned | 2021-06-01T14:03:51Z | |
dc.date.available | 2021-06-01T14:03:51Z | |
dc.date.issued | 2015-02 | |
dc.date.updated | 2021-06-01T14:03:50Z | |
dc.description.abstract | BackgroundLaser Doppler flowmetry (LDF) is widely used for estimating cerebral blood flow changes during intraluminal middle cerebral artery occlusion (MCAO). No investigation has systematically examined LDF efficacy in standardizing outcome. We examined MCAO histologic and behavioral outcome as a function of LDF measurement.Materials and methodsRats were subjected to 90min MCAO by 4 surgeons having different levels of MCAO surgical experience. LDF was measured in all rats during ischemia. By random assignment, LDF values were (Assisted) or were not (Blinded) made available to each surgeon during MCAO (n=12-17 per group). Neurologic and histologic outcomes were measured 7 days post-MCAO. A second study examined LDF effects on 1-day post-MCAO outcome.ResultsPooled across surgeons, intra-ischemic %LDF change (P=0.12), neurologic scores (Assisted vs. Blinded=14±6 vs. 13±7, P=0.61, mean±standard deviation) and cerebral infarct volume (162±63mm(3)vs. 143±86mm(3), P=0.24) were not different between groups. Only for one surgeon (novice) did LDF use alter infarct volume (145±28mm(3)vs. 98±61mm(3), P=0.03). LDF use decreased infarct volume coefficient of variation (COV) by 35% (P=0.02), but had no effect on neurologic score COV.Comparison with existing methodsWe compared intraluminal MCAO outcome as a function of LDF use.ConclusionsLDF measurement altered neither neurologic nor histologic MCAO outcome. LDF did not decrease neurologic deficit COV, but did decrease infarct volume COV. LDF may allow use of fewer animals if infarct volume is the primary dependent variable, but is unlikely to impact requisite sample sizes if neurologic function is of primary interest. | |
dc.identifier | S0165-0270(14)00419-1 | |
dc.identifier.issn | 0165-0270 | |
dc.identifier.issn | 1872-678X | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | Elsevier BV | |
dc.relation.ispartof | Journal of neuroscience methods | |
dc.relation.isversionof | 10.1016/j.jneumeth.2014.12.006 | |
dc.subject | Animals | |
dc.subject | Rats | |
dc.subject | Rats, Wistar | |
dc.subject | Infarction, Middle Cerebral Artery | |
dc.subject | Nylons | |
dc.subject | Laser-Doppler Flowmetry | |
dc.subject | Random Allocation | |
dc.subject | Single-Blind Method | |
dc.subject | Male | |
dc.title | A blinded randomized assessment of laser Doppler flowmetry efficacy in standardizing outcome from intraluminal filament MCAO in the rat. | |
dc.type | Journal article | |
duke.contributor.orcid | Sheng, Huaxin|0000-0002-4325-2940 | |
pubs.begin-page | 111 | |
pubs.end-page | 120 | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | Neurobiology | |
pubs.organisational-group | Duke Institute for Brain Sciences | |
pubs.organisational-group | Surgery | |
pubs.organisational-group | Anesthesiology, Neuroanesthesia | |
pubs.organisational-group | Duke | |
pubs.organisational-group | Basic Science Departments | |
pubs.organisational-group | University Institutes and Centers | |
pubs.organisational-group | Institutes and Provost's Academic Units | |
pubs.organisational-group | Clinical Science Departments | |
pubs.organisational-group | Anesthesiology | |
pubs.publication-status | Published | |
pubs.volume | 241 |