Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells.
| dc.contributor.author | Parker, Robert | |
| dc.contributor.author | Partridge, Thomas | |
| dc.contributor.author | Wormald, Catherine | |
| dc.contributor.author | Kawahara, Rebeca | |
| dc.contributor.author | Stalls, Victoria | |
| dc.contributor.author | Aggelakopoulou, Maria | |
| dc.contributor.author | Parker, Jimmy | |
| dc.contributor.author | Powell Doherty, Rebecca | |
| dc.contributor.author | Ariosa Morejon, Yoanna | |
| dc.contributor.author | Lee, Esther | |
| dc.contributor.author | Saunders, Kevin | |
| dc.contributor.author | Haynes, Barton F | |
| dc.contributor.author | Acharya, Priyamvada | |
| dc.contributor.author | Thaysen-Andersen, Morten | |
| dc.contributor.author | Borrow, Persephone | |
| dc.contributor.author | Ternette, Nicola | |
| dc.date.accessioned | 2021-06-01T13:17:24Z | |
| dc.date.available | 2021-06-01T13:17:24Z | |
| dc.date.issued | 2021-05-13 | |
| dc.date.updated | 2021-06-01T13:17:21Z | |
| dc.description.abstract | Understanding and eliciting protective immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an urgent priority. To facilitate these objectives, we profile the repertoire of human leukocyte antigen class II (HLA-II)-bound peptides presented by HLA-DR diverse monocyte-derived dendritic cells pulsed with SARS-CoV-2 spike (S) protein. We identify 209 unique HLA-II-bound peptide sequences, many forming nested sets, which map to sites throughout S including glycosylated regions. Comparison of the glycosylation profile of the S protein to that of the HLA-II-bound S peptides reveals substantial trimming of glycan residues on the latter, likely induced during antigen processing. Our data also highlight the receptor-binding motif in S1 as a HLA-DR-binding peptide-rich region and identify S2-derived peptides with potential for targeting by cross-protective vaccine-elicited responses. Results from this study will aid analysis of CD4+ T cell responses in infected individuals and vaccine recipients and have application in next-generation vaccine design. | |
| dc.identifier | S2211-1247(21)00524-6 | |
| dc.identifier.issn | 2211-1247 | |
| dc.identifier.issn | 2211-1247 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Elsevier BV | |
| dc.relation.ispartof | Cell reports | |
| dc.relation.isversionof | 10.1016/j.celrep.2021.109179 | |
| dc.subject | HLA class II | |
| dc.subject | HLA-II | |
| dc.subject | LC-MS | |
| dc.subject | SARS-CoV-2 | |
| dc.subject | antigen presentation | |
| dc.subject | dentritic cells | |
| dc.subject | glycopeptides | |
| dc.subject | glycoslyation | |
| dc.subject | human leukocyte antigen | |
| dc.subject | immunopeptidomics | |
| dc.title | Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Saunders, Kevin|0000-0001-7399-7954 | |
| pubs.begin-page | 109179 | |
| pubs.issue | 8 | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Duke Human Vaccine Institute | |
| pubs.organisational-group | Surgery, Surgical Sciences | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Surgery | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.publication-status | Published | |
| pubs.volume | 35 |
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