Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.

dc.contributor.author

Moutos, Franklin T

dc.contributor.author

Guilak, Farshid

dc.coverage.spatial

United States

dc.date.accessioned

2011-04-15T16:46:37Z

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2010-04

dc.description.abstract

Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear, and viscoelastic biomechanical characteristics of native cartilage as the basis for developing functional tissue-engineered constructs. Three-dimensionally woven poly(epsilon-caprolactone) (PCL) scaffolds were encapsulated with a fibrin hydrogel, seeded with human adipose-derived stem cells, and cultured for 28 days in chondrogenic culture conditions. Biomechanical testing showed that PCL-based constructs exhibited baseline compressive and shear properties similar to those of native cartilage and maintained these properties throughout the culture period, while supporting the synthesis of a collagen-rich extracellular matrix. Further, constructs displayed an equilibrium coefficient of friction similar to that of native articular cartilage (mu(eq) approximately 0.1-0.3) over the prescribed culture period. Our findings show that three-dimensionally woven PCL-fibrin composite scaffolds can be produced with cartilage-like mechanical properties, and that these engineered properties can be maintained in culture while seeded stem cells regenerate a new, functional tissue construct.

dc.description.version

Version of Record

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/19903085

dc.identifier.eissn

1937-335X

dc.identifier.uri

https://hdl.handle.net/10161/3355

dc.language

eng

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en_US

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Mary Ann Liebert Inc

dc.relation.ispartof

Tissue Eng Part A

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10.1089/ten.TEA.2009.0480

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Tissue Engineering Part a

dc.subject

Adult Stem Cells

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Biocompatible Materials

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Biomechanical Phenomena

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Cartilage, Articular

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Chondrocytes

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Chondrogenesis

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Collagen

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Compressive Strength

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Extracellular Matrix

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Fibrin

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Friction

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Humans

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Hydrogels

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Materials Testing

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Microscopy, Electron, Scanning

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Polyesters

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Regeneration

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Shear Strength

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Tensile Strength

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Tissue Engineering

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Tissue Scaffolds

dc.title

Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.

dc.type

Journal article

duke.date.pubdate

2010-4-0

duke.description.issue

4

duke.description.volume

16

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/19903085

pubs.begin-page

1291

pubs.end-page

1301

pubs.issue

4

pubs.organisational-group

Biomedical Engineering

pubs.organisational-group

Duke

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Pratt School of Engineering

pubs.publication-status

Published

pubs.volume

16

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