Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.
dc.contributor.author | Moutos, Franklin T | |
dc.contributor.author | Guilak, Farshid | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2011-04-15T16:46:37Z | |
dc.date.issued | 2010-04 | |
dc.description.abstract | Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear, and viscoelastic biomechanical characteristics of native cartilage as the basis for developing functional tissue-engineered constructs. Three-dimensionally woven poly(epsilon-caprolactone) (PCL) scaffolds were encapsulated with a fibrin hydrogel, seeded with human adipose-derived stem cells, and cultured for 28 days in chondrogenic culture conditions. Biomechanical testing showed that PCL-based constructs exhibited baseline compressive and shear properties similar to those of native cartilage and maintained these properties throughout the culture period, while supporting the synthesis of a collagen-rich extracellular matrix. Further, constructs displayed an equilibrium coefficient of friction similar to that of native articular cartilage (mu(eq) approximately 0.1-0.3) over the prescribed culture period. Our findings show that three-dimensionally woven PCL-fibrin composite scaffolds can be produced with cartilage-like mechanical properties, and that these engineered properties can be maintained in culture while seeded stem cells regenerate a new, functional tissue construct. | |
dc.description.version | Version of Record | |
dc.identifier | ||
dc.identifier.eissn | 1937-335X | |
dc.identifier.uri | ||
dc.language | eng | |
dc.language.iso | en_US | |
dc.publisher | Mary Ann Liebert Inc | |
dc.relation.ispartof | Tissue Eng Part A | |
dc.relation.isversionof | 10.1089/ten.TEA.2009.0480 | |
dc.relation.journal | Tissue Engineering Part a | |
dc.subject | Adult Stem Cells | |
dc.subject | Biocompatible Materials | |
dc.subject | Biomechanical Phenomena | |
dc.subject | Cartilage, Articular | |
dc.subject | Chondrocytes | |
dc.subject | Chondrogenesis | |
dc.subject | Collagen | |
dc.subject | Compressive Strength | |
dc.subject | Extracellular Matrix | |
dc.subject | Fibrin | |
dc.subject | Friction | |
dc.subject | Humans | |
dc.subject | Hydrogels | |
dc.subject | Materials Testing | |
dc.subject | Microscopy, Electron, Scanning | |
dc.subject | Polyesters | |
dc.subject | Regeneration | |
dc.subject | Shear Strength | |
dc.subject | Tensile Strength | |
dc.subject | Tissue Engineering | |
dc.subject | Tissue Scaffolds | |
dc.title | Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering. | |
dc.type | Journal article | |
duke.date.pubdate | 2010-4-0 | |
duke.description.issue | 4 | |
duke.description.volume | 16 | |
pubs.author-url | ||
pubs.begin-page | 1291 | |
pubs.end-page | 1301 | |
pubs.issue | 4 | |
pubs.organisational-group | Biomedical Engineering | |
pubs.organisational-group | Duke | |
pubs.organisational-group | Pratt School of Engineering | |
pubs.publication-status | Published | |
pubs.volume | 16 |