Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction.

Al-Emran, Hassan M

Krumkamp, Ralf

Dekker, Denise Myriam

Eibach, Daniel

Aaby, Peter

Adu-Sarkodie, Yaw

Ali, Mohammad

Rubach, Mathew P

Bjerregaard-Andersen, Morten

Crump, John A

Cruz Espinoza, Ligia Maria

Løfberg, Sandra Valborg

Gassama Sow, Amy

Hertz, Julian T

Im, Justin

Jaeger, Anna

Kabore, Leon Parfait

Konings, Frank

Meyer, Christian G

Niang, Aissatou

Pak, Gi Deok

Panzner, Ursula

Park, Se Eun

Rabezanahary, Henintsoa

Rakotozandrindrainy, Raphaël

Raminosoa, Tiana Mirana

Razafindrabe, Tsiriniaina Jean Luco

Sampo, Emmanuel

Schütt-Gerowitt, Heidi

Sarpong, Nimako

Soura, Abdramane Bassiahi

Tall, Adama

von Kalckreuth, Vera

Wierzba, Thomas F

May, Jürgen

Marks, Florian

United States

2017-03-02T18:53:59Z

2017-03-02T18:53:59Z

2016-03-15

Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi, 14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar, Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa could advance the knowledge of serotype distribution of Salmonella.

https://www.ncbi.nlm.nih.gov/pubmed/26933026

civ782

1537-6591

https://hdl.handle.net/10161/13757

eng

Oxford University Press (OUP)

Clin Infect Dis

10.1093/cid/civ782

PCR

Salmonella spp

serotyping

sub-Saharan Africa

Adolescent

Adult

Africa South of the Sahara

Child

Child, Preschool

Female

Humans

Infant

Infant, Newborn

Male

Multiplex Polymerase Chain Reaction

Salmonella Infections

Salmonella enterica

Serotyping

Young Adult

Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction.

Journal article

Hertz, Julian T|0000-0002-7396-4789

https://www.ncbi.nlm.nih.gov/pubmed/26933026

S80

S82

Clinical Science Departments

Duke

Global Health Institute

Institutes and Provost's Academic Units

Medicine

Medicine, Infectious Diseases

Pathology

School of Medicine

Surgery

Surgery, Emergency Medicine

University Institutes and Centers

Published

62 Suppl 1