Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells.
| dc.contributor.author | Wu, Mina | |
| dc.contributor.author | Yuan, Fan | |
| dc.coverage.spatial | United States | |
| dc.date.accessioned | 2011-06-30T18:48:11Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | Electric field mediated gene delivery or electrotransfection is a widely used method in various studies ranging from basic cell biology research to clinical gene therapy. Yet, mechanisms of electrotransfection are still controversial. To this end, we investigated the dependence of electrotransfection efficiency (eTE) on binding of plasmid DNA (pDNA) to plasma membrane and how treatment of cells with three endocytic inhibitors (chlorpromazine, genistein, dynasore) or silencing of dynamin expression with specific, small interfering RNA (siRNA) would affect the eTE. Our data demonstrated that the presence of divalent cations (Ca(2+) and Mg(2+)) in electrotransfection buffer enhanced pDNA adsorption to cell membrane and consequently, this enhanced adsorption led to an increase in eTE, up to a certain threshold concentration for each cation. Trypsin treatment of cells at 10 min post electrotransfection stripped off membrane-bound pDNA and resulted in a significant reduction in eTE, indicating that the time period for complete cellular uptake of pDNA (between 10 and 40 min) far exceeded the lifetime of electric field-induced transient pores (∼10 msec) in the cell membrane. Furthermore, treatment of cells with the siRNA and all three pharmacological inhibitors yielded substantial and statistically significant reductions in the eTE. These findings suggest that electrotransfection depends on two mechanisms: (i) binding of pDNA to cell membrane and (ii) endocytosis of membrane-bound pDNA. | |
| dc.description.sponsorship | This work was supported in part by a grant from the National Institutes of Health (NIH; CA94019). MW was supported in part by a NIH training grant for the Center for Biomolecular and Tissue Engineering at Duke University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | |
| dc.identifier | ||
| dc.identifier | PONE-D-11-05519 | |
| dc.identifier.eissn | 1932-6203 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.language.iso | en_US | |
| dc.publisher | Public Library of Science (PLoS) | |
| dc.relation.ispartof | PLoS One | |
| dc.relation.isversionof | 10.1371/journal.pone.0020923 | |
| dc.subject | Adsorption | |
| dc.subject | Animals | |
| dc.subject | Cations, Divalent | |
| dc.subject | Cell Line, Tumor | |
| dc.subject | Cell Membrane | |
| dc.subject | DNA | |
| dc.subject | Dynamins | |
| dc.subject | Electricity | |
| dc.subject | Endocytosis | |
| dc.subject | Gene Expression Regulation | |
| dc.subject | Intracellular Space | |
| dc.subject | Kinetics | |
| dc.subject | Mice | |
| dc.subject | Plasmids | |
| dc.subject | Transfection | |
| dc.title | Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells. | |
| dc.type | Journal article | |
| pubs.author-url | ||
| pubs.begin-page | e20923 | |
| pubs.issue | 6 | |
| pubs.organisational-group | Biomedical Engineering | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Ophthalmology | |
| pubs.organisational-group | Pratt School of Engineering | |
| pubs.organisational-group | School of Medicine | |
| pubs.publication-status | Published | |
| pubs.volume | 6 |