Association of an axonally transported polypeptide (H) with 100-A filaments. Use of immunoaffinity electron microscope grids.
| dc.contributor.author | Willard, M | |
| dc.contributor.author | Simon, C | |
| dc.contributor.author | Baitinger, C | |
| dc.contributor.author | Levine, J | |
| dc.contributor.author | Skene, P | |
| dc.date.accessioned | 2021-01-02T07:03:18Z | |
| dc.date.available | 2021-01-02T07:03:18Z | |
| dc.date.issued | 1980-06 | |
| dc.date.updated | 2021-01-02T07:03:16Z | |
| dc.description.abstract | Polypeptide H (mol wt 195,000) is axonally transported in rabbit retinal ganglion cells at a velocity of 0.7--1.1 mm/d, i.e., in the most slowly moving of the five transport groups described in these neurons. To identify the organelle with which H is associated, we purified H, prepared antibodies directed against it, and adsorbed the antibodies onto Formvar-coated electron microscope grids. When the resulting "immuno-affinity grids" were incubated with extracts of spinal cord and then examined in the electron microscope, they contained as many as 100 times more 100-A filaments than did grids coated similarly with nonimmune IgG. The ability of the anti-H IgG to specifically adsorb filaments to grids was completely blocked by incubating the IgG with polypeptide H. The 100-A filaments adsorbed to anti-H immunoaffinity grids could be specifically decorated by incubating them with anti-H IgG. These observations demonstrate that H antigens (and most likely H itself) are associated with 100-A neurofilaments. In addition, they suggest that the use of immunoaffinity grids may be a useful approach for determining the organelle associations of polypeptides. | |
| dc.identifier.issn | 0021-9525 | |
| dc.identifier.issn | 1540-8140 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Rockefeller University Press | |
| dc.relation.ispartof | The Journal of cell biology | |
| dc.relation.isversionof | 10.1083/jcb.85.3.587 | |
| dc.subject | Spinal Cord | |
| dc.subject | Cytoskeleton | |
| dc.subject | Animals | |
| dc.subject | Rabbits | |
| dc.subject | Nerve Tissue Proteins | |
| dc.subject | Microscopy, Electron | |
| dc.subject | Immunosorbent Techniques | |
| dc.subject | Axonal Transport | |
| dc.subject | Molecular Weight | |
| dc.title | Association of an axonally transported polypeptide (H) with 100-A filaments. Use of immunoaffinity electron microscope grids. | |
| dc.type | Journal article | |
| pubs.begin-page | 587 | |
| pubs.end-page | 596 | |
| pubs.issue | 3 | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Neurobiology | |
| pubs.organisational-group | Duke Science & Society | |
| pubs.organisational-group | Duke Institute for Brain Sciences | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Initiatives | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | University Institutes and Centers | |
| pubs.publication-status | Published | |
| pubs.volume | 85 |
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