Identification of late larval stage developmental checkpoints in Caenorhabditis elegans regulated by insulin/IGF and steroid hormone signaling pathways.

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2014-06

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Abstract

Organisms in the wild develop with varying food availability. During periods of nutritional scarcity, development may slow or arrest until conditions improve. The ability to modulate developmental programs in response to poor nutritional conditions requires a means of sensing the changing nutritional environment and limiting tissue growth. The mechanisms by which organisms accomplish this adaptation are not well understood. We sought to study this question by examining the effects of nutrient deprivation on Caenorhabditis elegans development during the late larval stages, L3 and L4, a period of extensive tissue growth and morphogenesis. By removing animals from food at different times, we show here that specific checkpoints exist in the early L3 and early L4 stages that systemically arrest the development of diverse tissues and cellular processes. These checkpoints occur once in each larval stage after molting and prior to initiation of the subsequent molting cycle. DAF-2, the insulin/insulin-like growth factor receptor, regulates passage through the L3 and L4 checkpoints in response to nutrition. The FOXO transcription factor DAF-16, a major target of insulin-like signaling, functions cell-nonautonomously in the hypodermis (skin) to arrest developmental upon nutrient removal. The effects of DAF-16 on progression through the L3 and L4 stages are mediated by DAF-9, a cytochrome P450 ortholog involved in the production of C. elegans steroid hormones. Our results identify a novel mode of C. elegans growth in which development progresses from one checkpoint to the next. At each checkpoint, nutritional conditions determine whether animals remain arrested or continue development to the next checkpoint.

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10.1371/journal.pgen.1004426

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Schindler, Adam J, L Ryan Baugh and David R Sherwood (2014). Identification of late larval stage developmental checkpoints in Caenorhabditis elegans regulated by insulin/IGF and steroid hormone signaling pathways. PLoS Genet, 10(6). p. e1004426. 10.1371/journal.pgen.1004426 Retrieved from https://hdl.handle.net/10161/10398.

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Scholars@Duke

Baugh

L. Ryan Baugh

Professor of Biology

The Baugh Lab is interested in phenotypic plasticity and physiological adaptation to variable environmental conditions. We are using the roundworm C. elegans to understand how animals adapt to starvation using primarily genetic and genomic approaches. We are studying how development is governed by nutrient availability, how animals survive starvation, and the long-term consequences of starvation including adult disease and transgenerational epigenetic inheritance.

Sherwood

David R. Sherwood

Jerry G. and Patricia Crawford Hubbard Professor

The Sherwood lab is interested in understanding mechanisms that drive dynamic cellular behaviors underlying normal development and human disease. We study 1) How cells invade into tissues, 2) How stem cells interact with their niches, and 3) How cells control and interact with extracellular matrix. Our lab primarily examines C. elegans development, in which simple cellular complexity, amenability to genetics/genomics/transgenics/molecular perturbations, and evolutionary comparisons facilitates powerful insights. One particular emphasis of our work is live-cell imaging, where we watch cellular behaviors and cell-extracellular matrix interactions unfold in real-time to understand their regulation and function.  Cell invasion, stem cell regulation, and cell-matrix interactions are fundamental to development, regeneration, cancer, and aging.  Our work aims to advance our understanding of these fascinating processes and positively influence human health.


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