Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.

Klein, Hannah L; Bačinskaja, Giedrė; Che, Jun; Cheblal, Anais; Elango, Rajula; Epshtein, Anastasiya; Fitzgerald, Devon M; Gómez-González, Belén; Khan, Sharik R; Kumar, Sandeep; Leland, Bryan A; Marie, Léa; Mei, Qian; Miné-Hattab, Judith; Piotrowska, Alicja; Polleys, Erica J; Putnam, Christopher D; Radchenko, Elina A; Saada, Anissia Ait; Sakofsky, Cynthia J; Shim, Eun Yong; Stracy, Mathew; Xia, Jun; Yan, Zhenxin; Yin, Yi; Aguilera, Andrés; Argueso, Juan Lucas; Freudenreich, Catherine H; Gasser, Susan M; Gordenin, Dmitry A; Haber, James E; Ira, Grzegorz; Jinks-Robertson, Sue; King, Megan C; Kolodner, Richard D; Kuzminov, Andrei; Lambert, Sarah Ae; Lee, Sang Eun; Miller, Kyle M; Mirkin, Sergei M; Petes, Thomas D; Rosenberg, Susan M; Rothstein, Rodney; Symington, Lorraine S; Zawadzki, Pawel; Kim, Nayun; Lisby, Michael; Malkova, Anna

Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.

dc.contributor.author	Klein, Hannah L
dc.contributor.author	Bačinskaja, Giedrė
dc.contributor.author	Che, Jun
dc.contributor.author	Cheblal, Anais
dc.contributor.author	Elango, Rajula
dc.contributor.author	Epshtein, Anastasiya
dc.contributor.author	Fitzgerald, Devon M
dc.contributor.author	Gómez-González, Belén
dc.contributor.author	Khan, Sharik R
dc.contributor.author	Kumar, Sandeep
dc.contributor.author	Leland, Bryan A
dc.contributor.author	Marie, Léa
dc.contributor.author	Mei, Qian
dc.contributor.author	Miné-Hattab, Judith
dc.contributor.author	Piotrowska, Alicja
dc.contributor.author	Polleys, Erica J
dc.contributor.author	Putnam, Christopher D
dc.contributor.author	Radchenko, Elina A
dc.contributor.author	Saada, Anissia Ait
dc.contributor.author	Sakofsky, Cynthia J
dc.contributor.author	Shim, Eun Yong
dc.contributor.author	Stracy, Mathew
dc.contributor.author	Xia, Jun
dc.contributor.author	Yan, Zhenxin
dc.contributor.author	Yin, Yi
dc.contributor.author	Aguilera, Andrés
dc.contributor.author	Argueso, Juan Lucas
dc.contributor.author	Freudenreich, Catherine H
dc.contributor.author	Gasser, Susan M
dc.contributor.author	Gordenin, Dmitry A
dc.contributor.author	Haber, James E
dc.contributor.author	Ira, Grzegorz
dc.contributor.author	Jinks-Robertson, Sue
dc.contributor.author	King, Megan C
dc.contributor.author	Kolodner, Richard D
dc.contributor.author	Kuzminov, Andrei
dc.contributor.author	Lambert, Sarah Ae
dc.contributor.author	Lee, Sang Eun
dc.contributor.author	Miller, Kyle M
dc.contributor.author	Mirkin, Sergei M
dc.contributor.author	Petes, Thomas D
dc.contributor.author	Rosenberg, Susan M
dc.contributor.author	Rothstein, Rodney
dc.contributor.author	Symington, Lorraine S
dc.contributor.author	Zawadzki, Pawel
dc.contributor.author	Kim, Nayun
dc.contributor.author	Lisby, Michael
dc.contributor.author	Malkova, Anna
dc.date.accessioned	2019-02-01T16:12:41Z
dc.date.available	2019-02-01T16:12:41Z
dc.date.issued	2019-01-07
dc.date.updated	2019-02-01T16:12:38Z
dc.description.abstract	Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
dc.identifier	MIC0178E138
dc.identifier.issn	2311-2638
dc.identifier.issn	2311-2638
dc.identifier.uri	https://hdl.handle.net/10161/18039
dc.language	eng
dc.publisher	Shared Science Publishers OG
dc.relation.ispartof	Microbial cell (Graz, Austria)
dc.relation.isversionof	10.15698/mic2019.01.664
dc.subject	DNA breaks
dc.subject	DNA repair centers
dc.subject	DNA resection
dc.subject	DSBs
dc.subject	Holliday junctions
dc.subject	R-loops
dc.subject	chromatin dynamics
dc.subject	chromosome rearrangements
dc.subject	crossovers
dc.subject	fluorescent proteins
dc.subject	gene amplification
dc.subject	gene conversion
dc.subject	genome instability
dc.subject	gross chromosome rearrangements
dc.subject	homologous recombination
dc.subject	mitotic recombination
dc.subject	mutagenesis
dc.subject	pulsed field gel electrophoresis
dc.subject	replication fork stalling
dc.subject	single-particle tracking
dc.subject	sister chromatid recombination
dc.subject	sister repetitive sequences
dc.subject	site-specific chromosome breaks
dc.subject	toxic recombination intermediates
dc.subject	yeast artificial chromosome
dc.title	Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.
dc.type	Journal article
duke.contributor.orcid	Jinks-Robertson, Sue\|0000-0001-5924-5852
duke.contributor.orcid	Petes, Thomas D\|0000-0003-4890-8002
pubs.begin-page	1
pubs.end-page	64
pubs.issue	1
pubs.organisational-group	School of Medicine
pubs.organisational-group	Duke
pubs.organisational-group	Duke Cancer Institute
pubs.organisational-group	Institutes and Centers
pubs.organisational-group	Molecular Genetics and Microbiology
pubs.organisational-group	Basic Science Departments
pubs.publication-status	Published
pubs.volume	6

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