NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.
| dc.contributor.author | Yang, Emily J | |
| dc.contributor.author | Yoo, Chan Yul | |
| dc.contributor.author | Liu, Jiangxin | |
| dc.contributor.author | Wang, He | |
| dc.contributor.author | Cao, Jun | |
| dc.contributor.author | Li, Fay-Wei | |
| dc.contributor.author | Pryer, Kathleen M | |
| dc.contributor.author | Sun, Tai-Ping | |
| dc.contributor.author | Weigel, Detlef | |
| dc.contributor.author | Zhou, Pei | |
| dc.contributor.author | Chen, Meng | |
| dc.date.accessioned | 2020-11-25T23:53:09Z | |
| dc.date.available | 2020-11-25T23:53:09Z | |
| dc.date.issued | 2019-06-14 | |
| dc.date.updated | 2020-11-25T23:53:06Z | |
| dc.description.abstract | Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG activation. NCP is dual-targeted to plastids and the nucleus. While nuclear NCP mediates the degradation of two repressors of chloroplast biogenesis, PIF1 and PIF3, NCP in plastids promotes the assembly of the PEP complex for PhAPG transcription. NCP and its paralog RCB are non-catalytic thioredoxin-like proteins that diverged in seed plants to adopt nonredundant functions in phytochrome signaling. These results support a model in which phytochromes control PhAPG expression through light-dependent double nuclear and plastidial switches that are linked by evolutionarily conserved and dual-localized regulatory proteins. | |
| dc.identifier | 10.1038/s41467-019-10517-1 | |
| dc.identifier.issn | 2041-1723 | |
| dc.identifier.issn | 2041-1723 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Springer Science and Business Media LLC | |
| dc.relation.ispartof | Nature communications | |
| dc.relation.isversionof | 10.1038/s41467-019-10517-1 | |
| dc.subject | Cell Nucleus | |
| dc.subject | Plastids | |
| dc.subject | Chloroplasts | |
| dc.subject | Plants, Genetically Modified | |
| dc.subject | Arabidopsis | |
| dc.subject | DNA-Directed RNA Polymerases | |
| dc.subject | Phytochrome | |
| dc.subject | Molecular Chaperones | |
| dc.subject | Arabidopsis Proteins | |
| dc.subject | Signal Transduction | |
| dc.subject | Photosynthesis | |
| dc.subject | Transcription, Genetic | |
| dc.subject | Gene Expression Regulation, Plant | |
| dc.subject | Light | |
| dc.subject | Basic Helix-Loop-Helix Transcription Factors | |
| dc.title | NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Pryer, Kathleen M|0000-0002-9776-6736 | |
| duke.contributor.orcid | Sun, Tai-Ping|0000-0001-5223-2936 | |
| duke.contributor.orcid | Zhou, Pei|0000-0002-7823-3416 | |
| pubs.begin-page | 2630 | |
| pubs.issue | 1 | |
| pubs.organisational-group | Trinity College of Arts & Sciences | |
| pubs.organisational-group | Biology | |
| pubs.organisational-group | Duke Science & Society | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Initiatives | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Biochemistry | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.publication-status | Published | |
| pubs.volume | 10 |
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