Use of 16S ribosomal RNA gene analyses to characterize the bacterial signature associated with poor oral health in West Virginia.
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2011-03-01
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BACKGROUND: West Virginia has the worst oral health in the United States, but the reasons for this are unclear. This pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease. METHODS: Bacteria in subgingival plaque samples from twelve participants in two independent West Virginia dental-related studies were characterized using 16S rRNA gene sequencing and Human Oral Microbe Identification Microarray (HOMIM) analysis. Unifrac analysis was used to characterize phylogenetic differences between bacterial communities obtained from plaque of participants with low or high oral disease, which was further evaluated using clustering and Principal Coordinate Analysis. RESULTS: Statistically different bacterial signatures (P<0.001) were identified in subgingival plaque of individuals with low or high oral disease in West Virginia based on 16S rRNA gene sequencing. Low disease contained a high frequency of Veillonella and Streptococcus, with a moderate number of Capnocytophaga. High disease exhibited substantially increased bacterial diversity and included a large proportion of Clostridiales cluster bacteria (Selenomonas, Eubacterium, Dialister). Phylogenetic trees constructed using 16S rRNA gene sequencing revealed that Clostridiales were repeated colonizers in plaque associated with high oral disease, providing evidence that the oral environment is somehow influencing the bacterial signature linked to disease. CONCLUSIONS: Culture-independent analyses identified an atypical bacterial signature associated with high oral disease in West Virginians and provided evidence that the oral environment influenced this signature. Both findings provide insight into the etiology of the oral disparity in West Virginia.
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Olson, Joan C, Christopher F Cuff, Slawomir Lukomski, Ewa Lukomska, Yeremi Canizales, Bei Wu, Richard J Crout, John G Thomas, et al. (2011). Use of 16S ribosomal RNA gene analyses to characterize the bacterial signature associated with poor oral health in West Virginia. BMC Oral Health, 11. p. 7. 10.1186/1472-6831-11-7 Retrieved from https://hdl.handle.net/10161/5876.
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Bei Wu
Bei Wu, PhD, is Pauline Gratz Professor of Nursing, Director for International Research at the School of Nursing, and a member of the Global Health Institute. She is also a senior fellow at the Center for the Study of Aging and Human Development. Dr. Wu’s areas of research expertise include aging and global health.
Dr. Wu received her M.S. and Ph.D. in gerontology from the University of Massachusetts, Boston. She is a fellow of the Gerontological Society of America (GSA) and the Association for Gerontology in Higher Education (AGHE). She is an internationally known expert on China’s long-term care policy and system development. She has worked closely with several schools of public health and schools of nursing in China on educational and research initiatives, such as conducting joint research projects, providing lectures, making presentations, and organizing professional conferences/training workshops. As the Principal Investigator, Dr. Wu has led many National Institutes of Health (NIH) and Center for Disease Control and Prevention (CDC) funded projects on aging and health related issues including long-term care, dementia, caregiving, oral health, and health services utilization. She is also the Methods Core Director for the recently NIH-funded center on Adaptive Leadership in Symptom Science.
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