Validation of an Animal Isolation Imaging Chamber for Use in Animal Biosafety Level-3 Containment.
| dc.contributor.author | Alderman, T Scott | |
| dc.contributor.author | Frothingham, Richard | |
| dc.contributor.author | Sempowski, Gregory D | |
| dc.date.accessioned | 2022-08-03T20:45:55Z | |
| dc.date.available | 2022-08-03T20:45:55Z | |
| dc.date.issued | 2010-01 | |
| dc.date.updated | 2022-08-03T20:45:54Z | |
| dc.description.abstract | Live imaging of animals infected with pathogenic microbes poses a contamination risk to equipment, personnel and other animals. A Caliper animal isolation chamber designed for the IVIS(®) Spectrum imaging system was tested as a containment device for mice infected with microbes assigned to animal biosafety level-3 (ABSL-3). A testing protocol was developed by adapting two published standards to test other equipment in high containment environments. The protocol included quantitative leak-testing of the high efficiency particulate air (HEPA) filters, soap bubble testing of the animal isolation chamber, and pressure decay testing of the complete containment system. HEPA filters were > 99.999% efficient (< 0.001% leakage). When attached to the Spectrum at the normal flow rate of oxygen/anesthetic mix (0.25 L/min), the chamber was positively pressurized at 0.11 inches of water (in H(2)O). No leaks were detected by soap bubble testing at flow rates of 0.25 L/min to 2.0 L/min, generating pressures up to 2.90 in H(2)O. (26-fold increase over normal operating pressure). The complete containment system passed pressure decay testing at 2.0 in H(2)O by sustaining 95% of the initial pressure over a 30 minute period.The Caliper animal isolation chamber provides appropriate isolation for the IVIS(®) Spectrum imaging system. When used as a containment device, it must undergo periodic performance testing, as described here, since it operates under positive pressure. The chamber is an appropriate component of ABSL-3 containment when combined with proper administrative controls and work practices. The testing protocol described here can be used to validate containment devices for other imaging systems or animal species. | |
| dc.identifier.issn | 1535-6760 | |
| dc.identifier.issn | 2470-1246 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Mary Ann Liebert Inc | |
| dc.relation.ispartof | Applied biosafety : journal of the American Biological Safety Association | |
| dc.relation.isversionof | 10.1177/153567601001500203 | |
| dc.title | Validation of an Animal Isolation Imaging Chamber for Use in Animal Biosafety Level-3 Containment. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Sempowski, Gregory D|0000-0003-0391-6594 | |
| pubs.begin-page | 62 | |
| pubs.end-page | 66 | |
| pubs.issue | 2 | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Faculty | |
| pubs.organisational-group | Staff | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Medicine | |
| pubs.organisational-group | Medicine, Duke Human Vaccine Institute | |
| pubs.organisational-group | Medicine, Infectious Diseases | |
| pubs.organisational-group | Duke Human Vaccine Institute | |
| pubs.publication-status | Published | |
| pubs.volume | 15 |