Nogo receptor 1 is expressed by nearly all retinal ganglion cells.

dc.contributor.author

Solomon, Alexander M

dc.contributor.author

Westbrook, Teleza

dc.contributor.author

Field, Greg D

dc.contributor.author

McGee, Aaron W

dc.date.accessioned

2019-01-03T15:39:35Z

dc.date.available

2019-01-03T15:39:35Z

dc.date.issued

2018-01

dc.date.updated

2019-01-03T15:39:28Z

dc.description.abstract

A variety of conditions ranging from glaucoma to blunt force trauma lead to optic nerve atrophy. Identifying signaling pathways for stimulating axon growth in the optic nerve may lead to treatments for these pathologies. Inhibiting signaling by the nogo-66 receptor 1 (NgR1) promotes the re-extension of axons following a crush injury to the optic nerve, and while NgR1 mRNA and protein expression are observed in the retinal ganglion cell (RGC) layer and inner nuclear layer, which retinal cell types express NgR1 remains unknown. Here we determine the expression pattern of NgR1 in the mouse retina by co-labeling neurons with characterized markers of specific retinal neurons together with antibodies specific for NgR1 or Green Fluorescent Protein expressed under control of the ngr1 promoter. We demonstrate that more than 99% of RGCs express NgR1. Thus, inhibiting NgR1 function may ubiquitously promote the regeneration of axons by RGCs. These results provide additional support for the therapeutic potential of NgR1 signaling in reversing optic nerve atrophy.

dc.identifier

PONE-D-17-27133

dc.identifier.issn

1932-6203

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1932-6203

dc.identifier.uri

https://hdl.handle.net/10161/17866

dc.language

eng

dc.publisher

Public Library of Science (PLoS)

dc.relation.ispartof

PloS one

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10.1371/journal.pone.0196565

dc.subject

Axons

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Retinal Ganglion Cells

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Optic Nerve

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Animals

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Mice, Transgenic

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Mice, Knockout

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Mice

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Optic Nerve Injuries

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Green Fluorescent Proteins

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RNA, Messenger

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Immunohistochemistry

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Nerve Regeneration

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Signal Transduction

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Gene Expression

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Nogo Receptor 1

dc.title

Nogo receptor 1 is expressed by nearly all retinal ganglion cells.

dc.type

Journal article

duke.contributor.orcid

Field, Greg D|0000-0001-5942-2679

pubs.begin-page

e0196565

pubs.issue

5

pubs.organisational-group

School of Medicine

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Duke

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Biomedical Engineering

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Pratt School of Engineering

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Neurobiology

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Basic Science Departments

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Duke Institute for Brain Sciences

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University Institutes and Centers

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Institutes and Provost's Academic Units

pubs.publication-status

Published

pubs.volume

13

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