Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.

Huang, Xin

Zauscher, Stefan

Klitzman, Bruce

Truskey, George A

Reichert, William M

Kenan, Daniel J

Grinstaff, Mark W

United States

2015-07-30T22:57:21Z

2010-06

Resorbable scaffolds such as polyglycolic acid (PGA) are employed in a number of clinical and tissue engineering applications owing to their desirable property of allowing remodeling to form native tissue over time. However, native PGA does not promote endothelial cell adhesion. Here we describe a novel treatment with hetero-bifunctional peptide linkers, termed "interfacial biomaterials" (IFBMs), which are used to alter the surface of PGA to provide appropriate biological cues. IFBMs couple an affinity peptide for the material with a biologically active peptide that promotes desired cellular responses. One such PGA affinity peptide was coupled to the integrin binding domain, Arg-Gly-Asp (RGD), to build a chemically synthesized bimodular 27 amino acid peptide that mediated interactions between PGA and integrin receptors on endothelial cells. Quartz crystal microbalance with dissipation monitoring (QCMD) was used to determine the association constant (K (A) 1 x 10(7) M(-1)) and surface thickness (~3.5 nm). Cell binding studies indicated that IFBM efficiently mediated adhesion, spreading, and cytoskeletal organization of endothelial cells on PGA in an integrin-dependent manner. We show that the IFBM peptide promotes a 200% increase in endothelial cell binding to PGA as well as 70-120% increase in cell spreading from 30 to 60 minutes after plating.

http://www.ncbi.nlm.nih.gov/pubmed/20300848

1573-9686

https://hdl.handle.net/10161/10344

eng

Springer Science and Business Media LLC

Ann Biomed Eng

10.1007/s10439-010-9986-5

Cell Adhesion

Cell Movement

Cells, Cultured

Coated Materials, Biocompatible

Endothelial Cells

Humans

Materials Testing

Oligopeptides

Polyglycolic Acid

Surface Properties

Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.

Journal article

Zauscher, Stefan|0000-0002-2290-7178

Truskey, George A|0000-0002-6885-4489

http://www.ncbi.nlm.nih.gov/pubmed/20300848

1965

1976

6

Basic Science Departments

Biomedical Engineering

Cell Biology

Chemistry

Clinical Science Departments

Duke

Duke Science & Society

Initiatives

Institutes and Provost's Academic Units

Mechanical Engineering and Materials Science

Pratt School of Engineering

School of Medicine

Surgery

Surgery, Plastic, Maxillofacial, and Oral Surgery

Trinity College of Arts & Sciences

Published

38