Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.

Abstract

To investigate the performance of a nucleic acid amplification test (NAAT) for the diagnosis of Mycobacterium tuberculosis bacteremia, 5-ml aliquots of blood were inoculated into bioMérieux mycobacterial (MB) bottles and incubated, and 5-ml aliquots of blood were extracted and tested by real-time PCR. Of 25 samples from patients with M. tuberculosis bacteremia, 9 (36.0%) were positive and 1 (1.5%) of 66 control samples was positive by NAAT. The NAAT shows promise, but modifications should focus on improving sensitivity.

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Citation

Published Version (Please cite this version)

10.1128/JCM.05963-11

Publication Info

Crump, John A, Marion J Tuohy, Anne B Morrissey, Habib O Ramadhani, Boniface N Njau, Venance P Maro, L Barth Reller, Gary W Procop, et al. (2012). Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. J Clin Microbiol, 50(1). pp. 138–141. 10.1128/JCM.05963-11 Retrieved from https://hdl.handle.net/10161/13790.

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Scholars@Duke

Crump

John Andrew Crump

Adjunct Professor in the Department of Medicine

I am an Adjunct Professor of Medicine, Pathology, and Global Health. My work with Duke University is primarily based in northern Tanzania where I am former Site Leader and current Principal Investigator on projects linked to Duke University’s collaborative research program at Kilimanjaro Christian Medical Centre. I oversee the design and implementation of research studies on infectious diseases, particularly febrile illness, invasive bacterial disease, zoonotic infections, and infectious diseases diagnostics. In addition, I am Professor of Medicine, Pathology, and Global Health at the University of Otago and a medical epidemiologist with the US Centers for Disease Control and Prevention (CDC). My CDC work focuses on non-malaria febrile illness.

Reller

Lyman Barth Reller

Professor of Pathology

To develop and to evaluate procedures and protocols that support, enhance, and extend the ability of the clinical laboratories to carry out effectively their primary service and teaching responsibilities as relates to detection of sepsis, antimicrobial susceptibility testing, and diagnosis of tuberculosis.


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