Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.

Mansukhani, A; Dell'Era, P; Moscatelli, D; Kornbluth, S; Hanafusa, H; Basilico, C

Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.

dc.contributor.author	Mansukhani, A
dc.contributor.author	Dell'Era, P
dc.contributor.author	Moscatelli, D
dc.contributor.author	Kornbluth, S
dc.contributor.author	Hanafusa, H
dc.contributor.author	Basilico, C
dc.coverage.spatial	United States
dc.date.accessioned	2014-03-13T17:02:20Z
dc.date.issued	1992-04-15
dc.description.abstract	The bek gene encodes a member of the high-affinity fibroblast growth factor receptor family. The BEK/FGFR-2 receptor is a membrane-spanning tyrosine kinase with the typical features of FGF receptors. We have cloned a murine bek cDNA and expressed it in receptor-negative Chinese hamster ovary cells and in 32D myeloid cells. The BEK receptor expressed in Chinese hamster ovary cells binds acidic FGF, basic FGF, and Kaposi FGF equally well but does not bind keratinocyte growth factor or FGF-5 appreciably. Upon treatment with basic FGF or Kaposi FGF, the BEK receptor is phosphorylated and a mitogenic response is achieved. Heparan sulfate proteoglycans have been shown to play an obligate role in basic FGF binding to the high-affinity FLG receptor. Unlike the BEK-expressing Chinese hamster ovary cells, 32D cells expressing the BEK receptor require the addition of exogenous heparin in order to grow in the presence of basic FGF or Kaposi FGF. We show that the addition of heparin greatly enhances the binding of radio-labeled basic FGF to the receptor. Thus the BEK receptor, like FLG, also requires an interaction with heparan sulfate proteoglycans to facilitate binding to its ligands.
dc.identifier	http://www.ncbi.nlm.nih.gov/pubmed/1373495
dc.identifier.issn	0027-8424
dc.identifier.uri	https://hdl.handle.net/10161/8400
dc.language	eng
dc.publisher	Proceedings of the National Academy of Sciences
dc.relation.ispartof	Proc Natl Acad Sci U S A
dc.subject	Amino Acid Sequence
dc.subject	Animals
dc.subject	Binding, Competitive
dc.subject	CHO Cells
dc.subject	Cell Division
dc.subject	Cell Line
dc.subject	Cloning, Molecular
dc.subject	Cricetinae
dc.subject	DNA
dc.subject	Fibroblast Growth Factor 1
dc.subject	Fibroblast Growth Factor 2
dc.subject	Fibroblast Growth Factors
dc.subject	Gene Library
dc.subject	Heparin
dc.subject	Kinetics
dc.subject	Male
dc.subject	Molecular Sequence Data
dc.subject	Protein-Tyrosine Kinases
dc.subject	Receptors, Cell Surface
dc.subject	Receptors, Fibroblast Growth Factor
dc.subject	Transfection
dc.title	Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.
dc.type	Journal article
pubs.author-url	http://www.ncbi.nlm.nih.gov/pubmed/1373495
pubs.begin-page	3305
pubs.end-page	3309
pubs.issue	8
pubs.organisational-group	Basic Science Departments
pubs.organisational-group	Duke
pubs.organisational-group	Duke Cancer Institute
pubs.organisational-group	Institutes and Centers
pubs.organisational-group	Pharmacology & Cancer Biology
pubs.organisational-group	School of Medicine
pubs.publication-status	Published
pubs.volume	89

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