Irisin - a myth rather than an exercise-inducible myokine.

dc.contributor.author

Albrecht, E

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Norheim, F

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Thiede, B

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Holen, T

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Ohashi, T

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Schering, L

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Lee, S

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Brenmoehl, J

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Thomas, S

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Drevon, CA

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Erickson, HP

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Maak, S

dc.coverage.spatial

England

dc.date.accessioned

2015-03-11T20:44:21Z

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2015-03-09

dc.description.abstract

The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at ~20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/25749243

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srep08889

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2045-2322

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https://hdl.handle.net/10161/9501

dc.language

eng

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Macmillan Publishers Limited. All rights reserved

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Sci Rep

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10.1038/srep08889

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Animals

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Artifacts

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Blood Chemical Analysis

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Cytokines

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Enzyme-Linked Immunosorbent Assay

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Exercise

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Fibronectins

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Humans

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Mice

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Muscle, Skeletal

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Reproducibility of Results

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Sensitivity and Specificity

dc.title

Irisin - a myth rather than an exercise-inducible myokine.

dc.type

Journal article

duke.contributor.orcid

Erickson, HP|0000-0002-9104-8987

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/25749243

pubs.begin-page

8889

pubs.organisational-group

Basic Science Departments

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Biochemistry

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Cell Biology

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Duke

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Duke Cancer Institute

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Institutes and Centers

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School of Medicine

pubs.publication-status

Published online

pubs.volume

5

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