The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.

Gong, Yiyang

England

2015-08-28T03:46:22Z

2015-08

Protein engineering over the past four years has made rhodopsin-based genetically encoded voltage indicators a leading candidate to achieve the task of reporting action potentials from a population of genetically targeted neurons in vivo. Rational design and large-scale screening efforts have steadily improved the dynamic range and kinetics of the rhodopsin voltage-sensing domain, and coupling these rhodopsins to bright fluorescent proteins has supported bright fluorescence readout of the large and rapid rhodopsin voltage response. The rhodopsin-fluorescent protein fusions have the highest achieved signal-to-noise ratios for detecting action potentials in neuronal cultures to date, and have successfully reported single spike events in vivo. Given the rapid pace of current development, the genetically encoded voltage indicator class is nearing the goal of robust spike imaging during live-animal behavioral experiments.

http://www.ncbi.nlm.nih.gov/pubmed/26143170

S1367-5931(15)00046-0

1879-0402

https://hdl.handle.net/10161/10439

eng

Elsevier BV

Curr Opin Chem Biol

10.1016/j.cbpa.2015.05.006

Action Potentials

Animals

Biosensing Techniques

Cells, Cultured

Fluorescence Resonance Energy Transfer

Fluorescent Dyes

Humans

Kinetics

Luminescent Proteins

Neurons

Recombinant Fusion Proteins

Rhodopsin

Voltage-Sensitive Dye Imaging

The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.

Journal article

http://www.ncbi.nlm.nih.gov/pubmed/26143170

84

89

Basic Science Departments

Biomedical Engineering

Duke

Duke Institute for Brain Sciences

Electrical and Computer Engineering

Institutes and Provost's Academic Units

Neurobiology

Pratt School of Engineering

School of Medicine

University Institutes and Centers

Published

27