A simple technique for augmentation of axonal ingrowth into chondroitinase-treated acellular nerve grafts using nerve growth factor.

Ovalle, Fernando; Patel, Ashit; Pollins, Alonda; de la Torre, Jorge; Vasconez, Luis; Hunt, Thomas R; Bucy, R Pat; Shack, R Bruce; Thayer, Wesley P

A simple technique for augmentation of axonal ingrowth into chondroitinase-treated acellular nerve grafts using nerve growth factor.

dc.contributor.author	Ovalle, Fernando
dc.contributor.author	Patel, Ashit
dc.contributor.author	Pollins, Alonda
dc.contributor.author	de la Torre, Jorge
dc.contributor.author	Vasconez, Luis
dc.contributor.author	Hunt, Thomas R
dc.contributor.author	Bucy, R Pat
dc.contributor.author	Shack, R Bruce
dc.contributor.author	Thayer, Wesley P
dc.date.accessioned	2023-04-03T11:51:50Z
dc.date.available	2023-04-03T11:51:50Z
dc.date.issued	2012-05
dc.date.updated	2023-04-03T11:51:50Z
dc.description.abstract	Background and purpose Improvement in axonal regeneration may lead to the development of longer nerve grafts and improved outcomes for patients with peripheral nerve injury. Although the use of acellular nerve grafts has been well documented (Groves et al, Exp Neurol. 2005;195:278-292; Krekoski et al, J Neurosci. 2001;21:6206-6213; Massey et al, Exp Neurol. 2008;209:426-445; Neubauer et al, Exp Neurol. 2007;207:163-170; Zuo et al, Exp Neurol. 2002;176:221-228), less is known about the ability of neurotrophic factors to enhance axonal regeneration. This study evaluates axonal ingrowth augmentation using acellular, chondroitinase-treated nerve grafts doped with nerve growth factor (NGF). Methods Acellular chondroitinase-treated murine nerve grafts were placed in experimental (NGF-treated grafts) and control (carrier-only grafts) rats. Five days after implantation, axonal regeneration was assessed by immunocytochemistry along with digital image analysis. Results Higher axon count was observed throughout the length of the nerve in the NGF group (P < 0.0001), peaking at 3 mm from proximal repair (P = 0.02). Although the NGF group displayed a higher axon count per slice, the mean diameter of individual NGF axons was smaller (P < 0.0001), potentially consistent with induction of sensory axons (Rich et al, J Neurocytol. 1987;16:261-268; Sofroniew et al, Annu Rev Neurosci. 2001;24:1217-1128; Yip et al, J Neurosci. 1984;4:2986-2992). Conclusion The simple technique of doping acellular, chondroitinase-treated nerve grafts with NGF can augment axonal ingrowth and possibly preferentially induce sensory axons.
dc.identifier.issn	0148-7043
dc.identifier.issn	1536-3708
dc.identifier.uri	https://hdl.handle.net/10161/26968
dc.publisher	Ovid Technologies (Wolters Kluwer Health)
dc.relation.ispartof	Annals of plastic surgery
dc.relation.isversionof	10.1097/sap.0b013e3182380974
dc.subject	Axons
dc.subject	Sciatic Nerve
dc.subject	Animals
dc.subject	Rats
dc.subject	Rats, Sprague-Dawley
dc.subject	Chondroitin ABC Lyase
dc.subject	Nerve Growth Factor
dc.subject	Enzyme-Linked Immunosorbent Assay
dc.subject	Guided Tissue Regeneration
dc.subject	Tissue Engineering
dc.subject	Nerve Regeneration
dc.subject	Female
dc.subject	Male
dc.subject	Peripheral Nerve Injuries
dc.title	A simple technique for augmentation of axonal ingrowth into chondroitinase-treated acellular nerve grafts using nerve growth factor.
dc.type	Conference
duke.contributor.orcid	Patel, Ashit\|0000-0002-8384-190X
pubs.begin-page	518
pubs.end-page	524
pubs.issue	5
pubs.organisational-group	Duke
pubs.organisational-group	School of Medicine
pubs.organisational-group	Clinical Science Departments
pubs.organisational-group	Surgery
pubs.organisational-group	Surgery, Plastic, Maxillofacial, and Oral Surgery
pubs.publication-status	Published
pubs.volume	68