A simple technique for augmentation of axonal ingrowth into chondroitinase-treated acellular nerve grafts using nerve growth factor.
dc.contributor.author | Ovalle, Fernando | |
dc.contributor.author | Patel, Ashit | |
dc.contributor.author | Pollins, Alonda | |
dc.contributor.author | de la Torre, Jorge | |
dc.contributor.author | Vasconez, Luis | |
dc.contributor.author | Hunt, Thomas R | |
dc.contributor.author | Bucy, R Pat | |
dc.contributor.author | Shack, R Bruce | |
dc.contributor.author | Thayer, Wesley P | |
dc.date.accessioned | 2023-04-03T11:51:50Z | |
dc.date.available | 2023-04-03T11:51:50Z | |
dc.date.issued | 2012-05 | |
dc.date.updated | 2023-04-03T11:51:50Z | |
dc.description.abstract | Background and purposeImprovement in axonal regeneration may lead to the development of longer nerve grafts and improved outcomes for patients with peripheral nerve injury. Although the use of acellular nerve grafts has been well documented (Groves et al, Exp Neurol. 2005;195:278-292; Krekoski et al, J Neurosci. 2001;21:6206-6213; Massey et al, Exp Neurol. 2008;209:426-445; Neubauer et al, Exp Neurol. 2007;207:163-170; Zuo et al, Exp Neurol. 2002;176:221-228), less is known about the ability of neurotrophic factors to enhance axonal regeneration. This study evaluates axonal ingrowth augmentation using acellular, chondroitinase-treated nerve grafts doped with nerve growth factor (NGF).MethodsAcellular chondroitinase-treated murine nerve grafts were placed in experimental (NGF-treated grafts) and control (carrier-only grafts) rats. Five days after implantation, axonal regeneration was assessed by immunocytochemistry along with digital image analysis.ResultsHigher axon count was observed throughout the length of the nerve in the NGF group (P < 0.0001), peaking at 3 mm from proximal repair (P = 0.02). Although the NGF group displayed a higher axon count per slice, the mean diameter of individual NGF axons was smaller (P < 0.0001), potentially consistent with induction of sensory axons (Rich et al, J Neurocytol. 1987;16:261-268; Sofroniew et al, Annu Rev Neurosci. 2001;24:1217-1128; Yip et al, J Neurosci. 1984;4:2986-2992).ConclusionThe simple technique of doping acellular, chondroitinase-treated nerve grafts with NGF can augment axonal ingrowth and possibly preferentially induce sensory axons. | |
dc.identifier.issn | 0148-7043 | |
dc.identifier.issn | 1536-3708 | |
dc.identifier.uri | ||
dc.publisher | Ovid Technologies (Wolters Kluwer Health) | |
dc.relation.ispartof | Annals of plastic surgery | |
dc.relation.isversionof | 10.1097/sap.0b013e3182380974 | |
dc.subject | Axons | |
dc.subject | Sciatic Nerve | |
dc.subject | Animals | |
dc.subject | Rats | |
dc.subject | Rats, Sprague-Dawley | |
dc.subject | Chondroitin ABC Lyase | |
dc.subject | Nerve Growth Factor | |
dc.subject | Enzyme-Linked Immunosorbent Assay | |
dc.subject | Guided Tissue Regeneration | |
dc.subject | Tissue Engineering | |
dc.subject | Nerve Regeneration | |
dc.subject | Female | |
dc.subject | Male | |
dc.subject | Peripheral Nerve Injuries | |
dc.title | A simple technique for augmentation of axonal ingrowth into chondroitinase-treated acellular nerve grafts using nerve growth factor. | |
dc.type | Conference | |
duke.contributor.orcid | Patel, Ashit|0000-0002-8384-190X | |
pubs.begin-page | 518 | |
pubs.end-page | 524 | |
pubs.issue | 5 | |
pubs.organisational-group | Duke | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | Clinical Science Departments | |
pubs.organisational-group | Surgery | |
pubs.organisational-group | Surgery, Plastic, Maxillofacial, and Oral Surgery | |
pubs.publication-status | Published | |
pubs.volume | 68 |
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