The potential repertoire of the innate immune system in the bladder: expression of pattern recognition receptors in the rat bladder and a rat urothelial cell line (MYP3 cells).

dc.contributor.author

Hughes, Francis M

dc.contributor.author

Turner, David P

dc.contributor.author

Todd Purves, J

dc.date.accessioned

2018-06-26T13:45:50Z

dc.date.available

2018-06-26T13:45:50Z

dc.date.issued

2015-12

dc.date.updated

2018-06-26T13:45:45Z

dc.description.abstract

The urothelium is a frontline sensor of the lower urinary tract, sampling the bladder lumen and stimulating an immune response to infectious and noxious agents. Pattern recognition receptors (PRRs) recognize such agents and coordinate the innate response, often by forming inflammasomes that activate caspase-1 and the release of interleukin-1. We have shown the presence of one PRR (NLRP3) in the urothelia and its central role in the inflammatory response to cyclophosphamide. The purpose of this study was to (1) assess the likely range of the PPR response by assessing the repertoire present in the rat bladder and (2) determine the utility of the MYP3 rat urothelia cell line for in vitro studies by assessing its PPR repertoire and functional responsiveness.Immunohistochemistry was performed for seven PPRs (NLRP1, NLRP3, NLRP6, NLRP7, NLRP12, NLRC4 and AIM2) on bladder sections and MYP3 cells. For functionality, MYP3 cells were challenged with the quintessential NLRP3 activator ATP and assessed for caspase-1 activation.All PPRs examined were expressed in the bladder and localized to the urothelial layer with several also in the detrusor (none in the interstitia). MYP3 cells also expressed all PRRs with a variable intracellular location. ATP-stimulated caspase-1 activity in MYP3 cells in a dose-dependent manner was reduced by knockdown of NLRP3 expression.The results suggest that the bladder possesses the capacity to initiate an innate immune response to a wide array of uropathological agents and the MYP3 cells will provide an excellent investigational tool for this field.

dc.identifier.issn

0301-1623

dc.identifier.issn

1573-2584

dc.identifier.uri

https://hdl.handle.net/10161/17177

dc.language

eng

dc.publisher

Springer Science and Business Media LLC

dc.relation.ispartof

International urology and nephrology

dc.relation.isversionof

10.1007/s11255-015-1126-6

dc.subject

Urothelium

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Cell Line

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Animals

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Rats

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Rats, Sprague-Dawley

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Caspase 1

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Carrier Proteins

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DNA-Binding Proteins

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Receptors, Cell Surface

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Receptors, Angiotensin

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Receptors, Vasopressin

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Nerve Tissue Proteins

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RNA, Small Interfering

dc.subject

Adenosine Triphosphate

dc.subject

Female

dc.subject

Receptors, Pattern Recognition

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Urinary Bladder

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Immunity, Innate

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Gene Knockdown Techniques

dc.title

The potential repertoire of the innate immune system in the bladder: expression of pattern recognition receptors in the rat bladder and a rat urothelial cell line (MYP3 cells).

dc.type

Journal article

duke.contributor.orcid

Hughes, Francis M|0000-0003-3776-3653

pubs.begin-page

1953

pubs.end-page

1964

pubs.issue

12

pubs.organisational-group

Staff

pubs.organisational-group

Duke

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School of Medicine

pubs.organisational-group

Surgery, Urology

pubs.organisational-group

Surgery

pubs.organisational-group

Clinical Science Departments

pubs.publication-status

Published

pubs.volume

47

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