Sperm DNA methylation altered by THC and nicotine: Vulnerability of neurodevelopmental genes with bivalent chromatin.
| dc.contributor.author | Schrott, Rose | |
| dc.contributor.author | Rajavel, Maya | |
| dc.contributor.author | Acharya, Kelly | |
| dc.contributor.author | Huang, Zhiqing | |
| dc.contributor.author | Acharya, Chaitanya | |
| dc.contributor.author | Hawkey, Andrew | |
| dc.contributor.author | Pippen, Erica | |
| dc.contributor.author | Lyerly, H Kim | |
| dc.contributor.author | Levin, Edward D | |
| dc.contributor.author | Murphy, Susan K | |
| dc.date.accessioned | 2023-07-01T14:03:49Z | |
| dc.date.available | 2023-07-01T14:03:49Z | |
| dc.date.issued | 2020-09 | |
| dc.date.updated | 2023-07-01T14:03:47Z | |
| dc.description.abstract | Men consume the most nicotine and cannabis products but impacts on sperm epigenetics are poorly characterized. Evidence suggests that preconception exposure to these drugs alters offspring neurodevelopment. Epigenetics may in part facilitate heritability. We therefore compared effects of exposure to tetrahydrocannabinol (THC) and nicotine on DNA methylation in rat sperm at genes involved in neurodevelopment. Reduced representation bisulfite sequencing data from sperm of rats exposed to THC via oral gavage showed that seven neurodevelopmentally active genes were significantly differentially methylated versus controls. Pyrosequencing data revealed majority overlap in differential methylation in sperm from rats exposed to THC via injection as well as those exposed to nicotine. Neurodevelopmental genes including autism candidates are vulnerable to environmental exposures and common features may mediate this vulnerability. We discovered that autism candidate genes are significantly enriched for bivalent chromatin structure, suggesting this configuration may increase vulnerability of genes in sperm to disrupted methylation. | |
| dc.identifier | 10.1038/s41598-020-72783-0 | |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Springer Science and Business Media LLC | |
| dc.relation.ispartof | Scientific reports | |
| dc.relation.isversionof | 10.1038/s41598-020-72783-0 | |
| dc.subject | Spermatozoa | |
| dc.subject | Chromatin | |
| dc.subject | Animals | |
| dc.subject | Rats | |
| dc.subject | Disease Models, Animal | |
| dc.subject | Nicotine | |
| dc.subject | Sequence Analysis, DNA | |
| dc.subject | Autistic Disorder | |
| dc.subject | DNA Methylation | |
| dc.subject | Epigenesis, Genetic | |
| dc.subject | CpG Islands | |
| dc.subject | Male | |
| dc.subject | Gene Regulatory Networks | |
| dc.subject | High-Throughput Nucleotide Sequencing | |
| dc.subject | Dronabinol | |
| dc.title | Sperm DNA methylation altered by THC and nicotine: Vulnerability of neurodevelopmental genes with bivalent chromatin. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Acharya, Kelly|0000-0003-1891-4196 | |
| duke.contributor.orcid | Acharya, Chaitanya|0000-0001-7149-1749 | |
| duke.contributor.orcid | Lyerly, H Kim|0000-0002-0063-4770 | |
| duke.contributor.orcid | Levin, Edward D|0000-0002-5060-9602 | |
| duke.contributor.orcid | Murphy, Susan K|0000-0001-8298-7272 | |
| pubs.begin-page | 16022 | |
| pubs.issue | 1 | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Nicholas School of the Environment | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Trinity College of Arts & Sciences | |
| pubs.organisational-group | Staff | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Integrative Immunobiology | |
| pubs.organisational-group | Pharmacology & Cancer Biology | |
| pubs.organisational-group | Obstetrics and Gynecology | |
| pubs.organisational-group | Pathology | |
| pubs.organisational-group | Psychiatry & Behavioral Sciences | |
| pubs.organisational-group | Surgery | |
| pubs.organisational-group | Obstetrics and Gynecology, Reproductive Endocrinology & Fertility | |
| pubs.organisational-group | Surgery, Surgical Sciences | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Psychology & Neuroscience | |
| pubs.organisational-group | Environmental Sciences and Policy | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | University Institutes and Centers | |
| pubs.organisational-group | Duke Global Health Institute | |
| pubs.organisational-group | Duke Institute for Brain Sciences | |
| pubs.organisational-group | Initiatives | |
| pubs.organisational-group | Duke Science & Society | |
| pubs.organisational-group | Psychiatry & Behavioral Sciences, Behavioral Medicine & Neurosciences | |
| pubs.organisational-group | Regeneration Next Initiative | |
| pubs.organisational-group | Obstetrics and Gynecology, Reproductive Sciences | |
| pubs.organisational-group | Duke - Margolis Center For Health Policy | |
| pubs.publication-status | Published | |
| pubs.volume | 10 |
Files
Original bundle
- Name:
- Sperm DNA methylation altered by THC and nicotine Vulnerability of neurodevelopmental genes with bivalent chromatin.pdf
- Size:
- 1.4 MB
- Format:
- Adobe Portable Document Format