Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera.

dc.contributor.author

Rinehart, Matthew T

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Shaked, Natan T

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Jenness, Nathan J

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Clark, Robert L

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Wax, Adam

dc.coverage.spatial

United States

dc.date.accessioned

2011-06-21T17:27:41Z

dc.date.issued

2010-08-01

dc.description.abstract

We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algorithm to extend the range of the optical path delay measurements that can be detected using a single temporal acquisition. We experimentally demonstrate this technique by acquiring the phase profiles of optically clear microstructures without 2pi ambiguities. In addition, the phase noise contribution arising from spectral channel crosstalk on the color camera is quantified.

dc.description.version

Version of Record

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/20680075

dc.identifier

204170

dc.identifier.eissn

1539-4794

dc.identifier.uri

https://hdl.handle.net/10161/4224

dc.language

eng

dc.language.iso

en_US

dc.publisher

The Optical Society

dc.relation.ispartof

Opt Lett

dc.relation.journal

Optics Letters

dc.subject

Algorithms

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Animals

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Humans

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Image Processing, Computer-Assisted

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Microfluidics

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Microscopy

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Microscopy, Electron, Scanning

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Optics and Photonics

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Refractometry

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Reproducibility of Results

dc.title

Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera.

dc.title.alternative
dc.type

Journal article

duke.date.pubdate

2010-8-1

duke.description.issue

15

duke.description.volume

35

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/20680075

pubs.begin-page

2612

pubs.end-page

2614

pubs.issue

15

pubs.organisational-group

Biomedical Engineering

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Duke

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Duke Institute for Brain Sciences

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Institutes and Provost's Academic Units

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Pratt School of Engineering

pubs.organisational-group

University Institutes and Centers

pubs.publication-status

Published

pubs.volume

35

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