ACE-inhibition increases podocyte number in experimental glomerular disease independent of proliferation.

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2015-06

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Abstract

Objective

The objective of this article is to test the effects of angiotensin-converting enzyme (ACE)-inhibition on glomerular epithelial cell number in an inducible experimental model of focal segmental glomerulosclerosis (FSGS).

Background

Although ACE-inhibition has been shown to limit podocyte loss by enhancing survival, little is known about its effect on podocyte number following an abrupt decline in disease.

Methods

Experimental FSGS was induced with cytotoxic antipodocyte antibody. Following induction, groups were randomized to receive the ACE-inhibitor enalapril, the smooth muscle relaxant hydralazine (blood pressure control) or drinking water. Blood pressure, kidney function and histology were measured seven and 14 days following disease induction.

Results

Both glomerulosclerosis and urinary albumin-to-creatinine ratio were less in the ACE-inhibition arm at day 14. At day 7 of disease, mean podocyte numbers were 26% and 29% lower in the enalapril and hydralazine arms, respectively, compared to normal mice in which no antibody was injected. At day 14, the mean podocyte number was only 18% lower in the enalapril arm, but was 39% lower in the hydralazine arm compared to normal mice. Podocyte proliferation did not occur at any time in any group. Compared to water- or hydralazine-treated mice with FSGS, the enalapril arm had a higher mean number of glomerular parietal epithelial cells that co-expressed the podocyte proteins WT-1 and synaptopodin, as well as phospho-ERK.

Conclusion

The results show following an abrupt decline in podocyte number, the initiation of ACE-inhibition but not hydralazine, was accompanied by higher podocyte number in the absence of proliferation. This was accompanied by a higher number of parietal epithelial cells that co-express podocyte proteins. Increasing podocyte number appears to be accompanied by reduced glomerulosclerosis.

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Epithelial Cells, Animals, Mice, Microfilament Proteins, Extracellular Signal-Regulated MAP Kinases, Enalapril, WT1 Proteins, Repressor Proteins, Antibodies, Angiotensin-Converting Enzyme Inhibitors, Endpoint Determination, Cell Count, Cell Proliferation, Protein Binding, Phosphorylation, Blood Pressure, Systole, Podocytes, PAX2 Transcription Factor, Glomerulosclerosis, Focal Segmental

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https://hdl.handle.net/10161/26502

Published Version (Please cite this version)

10.1177/1470320314543910

Publication Info

Zhang, Jiong, David Yanez, Anna Floege, Julia Lichtnekert, Ronald D Krofft, Zhi-Hong Liu, Jeffrey W Pippin, Stuart J Shankland, et al. (2015). ACE-inhibition increases podocyte number in experimental glomerular disease independent of proliferation. Journal of the renin-angiotensin-aldosterone system : JRAAS, 16(2). pp. 234–248. 10.1177/1470320314543910 Retrieved from https://hdl.handle.net/10161/26502.

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David Yanez

Professor of Biostatistics & Bioinformatics

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