Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.

dc.contributor.author

Yao, Junjie

dc.contributor.author

Kaberniuk, Andrii A

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Li, Lei

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Shcherbakova, Daria M

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Zhang, Ruiying

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Wang, Lidai

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Li, Guo

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Verkhusha, Vladislav V

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Wang, Lihong V

dc.coverage.spatial

United States

dc.date.accessioned

2016-09-14T20:31:28Z

dc.date.issued

2016-01

dc.description.abstract

Photoacoustic tomography (PAT) of genetically encoded probes allows for imaging of targeted biological processes deep in tissues with high spatial resolution; however, high background signals from blood can limit the achievable detection sensitivity. Here we describe a reversibly switchable nonfluorescent bacterial phytochrome for use in multiscale photoacoustic imaging, BphP1, with the most red-shifted absorption among genetically encoded probes. BphP1 binds a heme-derived biliverdin chromophore and is reversibly photoconvertible between red and near-infrared light-absorption states. We combined single-wavelength PAT with efficient BphP1 photoswitching, which enabled differential imaging with substantially decreased background signals, enhanced detection sensitivity, increased penetration depth and improved spatial resolution. We monitored tumor growth and metastasis with ∼ 100-μm resolution at depths approaching 10 mm using photoacoustic computed tomography, and we imaged individual cancer cells with a suboptical-diffraction resolution of ∼ 140 nm using photoacoustic microscopy. This technology is promising for biomedical studies at several scales.

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/26550774

dc.identifier

nmeth.3656

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1548-7105

dc.identifier.uri

https://hdl.handle.net/10161/12772

dc.language

eng

dc.publisher

Springer Science and Business Media LLC

dc.relation.ispartof

Nat Methods

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10.1038/nmeth.3656

dc.subject

Acoustics

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Animals

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Cell Line, Tumor

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Humans

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Mice

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Phytochrome

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Tomography

dc.title

Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.

dc.type

Journal article

duke.contributor.orcid

Yao, Junjie|0000-0002-2381-706X

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/26550774

pubs.begin-page

67

pubs.end-page

73

pubs.issue

1

pubs.organisational-group

Biomedical Engineering

pubs.organisational-group

Duke

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Pratt School of Engineering

pubs.publication-status

Published

pubs.volume

13

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