Cryptococcal cell morphology affects host cell interactions and pathogenicity.

dc.contributor.author

Okagaki, Laura H

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Strain, Anna K

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Nielsen, Judith N

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Charlier, Caroline

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Baltes, Nicholas J

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Chrétien, Fabrice

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Heitman, Joseph

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Dromer, Françoise

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Nielsen, Kirsten

dc.coverage.spatial

United States

dc.date.accessioned

2011-06-21T17:32:23Z

dc.date.issued

2010-06-17

dc.description.abstract

Cryptococcus neoformans is a common life-threatening human fungal pathogen. The size of cryptococcal cells is typically 5 to 10 microm. Cell enlargement was observed in vivo, producing cells up to 100 microm. These morphological changes in cell size affected pathogenicity via reducing phagocytosis by host mononuclear cells, increasing resistance to oxidative and nitrosative stress, and correlated with reduced penetration of the central nervous system. Cell enlargement was stimulated by coinfection with strains of opposite mating type, and ste3aDelta pheromone receptor mutant strains had reduced cell enlargement. Finally, analysis of DNA content in this novel cell type revealed that these enlarged cells were polyploid, uninucleate, and produced daughter cells in vivo. These results describe a novel mechanism by which C. neoformans evades host phagocytosis to allow survival of a subset of the population at early stages of infection. Thus, morphological changes play unique and specialized roles during infection.

dc.description.version

Version of Record

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/20585559

dc.identifier.eissn

1553-7374

dc.identifier.uri

https://hdl.handle.net/10161/4602

dc.language

eng

dc.language.iso

en_US

dc.publisher

Public Library of Science (PLoS)

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PLoS Pathog

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10.1371/journal.ppat.1000953

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Plos Pathogens

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Animals

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Blood-Brain Barrier

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Blotting, Western

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Brain

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Bronchoalveolar Lavage

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Cell Adhesion

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Cell Proliferation

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Cryptococcosis

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Cryptococcus neoformans

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Female

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Flow Cytometry

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Humans

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Lung Diseases, Fungal

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Mice

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Mice, Inbred A

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Oxidative Stress

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Phagocytosis

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Ploidies

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RNA, Messenger

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Receptors, Pheromone

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Reverse Transcriptase Polymerase Chain Reaction

dc.title

Cryptococcal cell morphology affects host cell interactions and pathogenicity.

dc.title.alternative
dc.type

Journal article

duke.date.pubdate

2010-6-0

duke.description.issue

6

duke.description.volume

6

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/20585559

pubs.begin-page

e1000953

pubs.issue

6

pubs.organisational-group

Basic Science Departments

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Clinical Science Departments

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Duke

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Duke Cancer Institute

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Institutes and Centers

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Medicine

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Medicine, Infectious Diseases

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Molecular Genetics and Microbiology

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Pharmacology & Cancer Biology

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School of Medicine

pubs.publication-status

Published online

pubs.volume

6

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