COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray.

dc.contributor.author

Heggestad, Jacob T

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Britton, Rhett J

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Kinnamon, David S

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Liu, Jason

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Anderson, Jack G

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Joh, Daniel Y

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Quinn, Zachary

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Fontes, Cassio M

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Hucknall, Angus M

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Parks, Robert

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Sempowski, Gregory D

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Denny, Thomas N

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Burke, Thomas W

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Haynes, Barton F

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Woods, Christopher W

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Chilkoti, Ashutosh

dc.date.accessioned

2023-04-04T12:57:59Z

dc.date.available

2023-04-04T12:57:59Z

dc.date.issued

2023-03

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2023-04-04T12:57:58Z

dc.description.abstract

Antigen tests to detect SARS-CoV-2 have emerged as a promising rapid diagnostic method for COVID-19, but they are unable to differentiate between variants of concern (VOCs). Here, we report a rapid point-of-care test (POC-T), termed CoVariant-SPOT, that uses a set of antibodies that are either tolerant or intolerant to spike protein mutations to identify the likely SARS-CoV-2 strain concurrent with COVID-19 diagnosis using antibodies targeting the nucleocapsid protein. All reagents are incorporated into a portable, multiplexed, and sensitive diagnostic platform built upon a nonfouling polymer brush. To validate CoVariant-SPOT, we tested recombinant SARS-CoV-2 proteins, inactivated viruses, and nasopharyngeal swab samples from COVID-19 positive and negative individuals and showed that CoVariant-SPOT can readily distinguish between two VOCs: Delta and Omicron. We believe that CoVariant-SPOT can serve as a valuable adjunct to next-generation sequencing to rapidly identify variants using a scalable and deployable POC-T, thereby enhancing community surveillance efforts worldwide and informing treatment selection.

dc.identifier.issn

0003-2700

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1520-6882

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https://hdl.handle.net/10161/26998

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eng

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American Chemical Society (ACS)

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Analytical chemistry

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10.1021/acs.analchem.2c05180

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COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray.

dc.type

Journal article

duke.contributor.orcid

Fontes, Cassio M|0000-0003-3157-478X

duke.contributor.orcid

Woods, Christopher W|0000-0001-7240-2453

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Duke

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School of Medicine

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Clinical Science Departments

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Institutes and Centers

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Medicine

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Medicine, Duke Human Vaccine Institute

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Duke Human Vaccine Institute

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Institutes and Provost's Academic Units

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University Institutes and Centers

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Duke Global Health Institute

pubs.publication-status

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