COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray.
dc.contributor.author | Heggestad, Jacob T | |
dc.contributor.author | Britton, Rhett J | |
dc.contributor.author | Kinnamon, David S | |
dc.contributor.author | Liu, Jason | |
dc.contributor.author | Anderson, Jack G | |
dc.contributor.author | Joh, Daniel Y | |
dc.contributor.author | Quinn, Zachary | |
dc.contributor.author | Fontes, Cassio M | |
dc.contributor.author | Hucknall, Angus M | |
dc.contributor.author | Parks, Robert | |
dc.contributor.author | Sempowski, Gregory D | |
dc.contributor.author | Denny, Thomas N | |
dc.contributor.author | Burke, Thomas W | |
dc.contributor.author | Haynes, Barton F | |
dc.contributor.author | Woods, Christopher W | |
dc.contributor.author | Chilkoti, Ashutosh | |
dc.date.accessioned | 2023-04-04T12:57:59Z | |
dc.date.available | 2023-04-04T12:57:59Z | |
dc.date.issued | 2023-03 | |
dc.date.updated | 2023-04-04T12:57:58Z | |
dc.description.abstract | Antigen tests to detect SARS-CoV-2 have emerged as a promising rapid diagnostic method for COVID-19, but they are unable to differentiate between variants of concern (VOCs). Here, we report a rapid point-of-care test (POC-T), termed CoVariant-SPOT, that uses a set of antibodies that are either tolerant or intolerant to spike protein mutations to identify the likely SARS-CoV-2 strain concurrent with COVID-19 diagnosis using antibodies targeting the nucleocapsid protein. All reagents are incorporated into a portable, multiplexed, and sensitive diagnostic platform built upon a nonfouling polymer brush. To validate CoVariant-SPOT, we tested recombinant SARS-CoV-2 proteins, inactivated viruses, and nasopharyngeal swab samples from COVID-19 positive and negative individuals and showed that CoVariant-SPOT can readily distinguish between two VOCs: Delta and Omicron. We believe that CoVariant-SPOT can serve as a valuable adjunct to next-generation sequencing to rapidly identify variants using a scalable and deployable POC-T, thereby enhancing community surveillance efforts worldwide and informing treatment selection. | |
dc.identifier.issn | 0003-2700 | |
dc.identifier.issn | 1520-6882 | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | American Chemical Society (ACS) | |
dc.relation.ispartof | Analytical chemistry | |
dc.relation.isversionof | 10.1021/acs.analchem.2c05180 | |
dc.title | COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray. | |
dc.type | Journal article | |
duke.contributor.orcid | Fontes, Cassio M|0000-0003-3157-478X | |
duke.contributor.orcid | Woods, Christopher W|0000-0001-7240-2453 | |
pubs.organisational-group | Duke | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | Clinical Science Departments | |
pubs.organisational-group | Institutes and Centers | |
pubs.organisational-group | Medicine | |
pubs.organisational-group | Medicine, Duke Human Vaccine Institute | |
pubs.organisational-group | Duke Human Vaccine Institute | |
pubs.organisational-group | Institutes and Provost's Academic Units | |
pubs.organisational-group | University Institutes and Centers | |
pubs.organisational-group | Duke Global Health Institute | |
pubs.publication-status | Published |