Anatomical and Functional Consequences of Microneedle Perforation of Round Window Membrane.

dc.contributor.author

Yu, Michelle

dc.contributor.author

Arteaga, Daniel N

dc.contributor.author

Aksit, Aykut

dc.contributor.author

Chiang, Harry

dc.contributor.author

Olson, Elizabeth S

dc.contributor.author

Kysar, Jeffrey W

dc.contributor.author

Lalwani, Anil K

dc.date.accessioned

2023-10-22T01:17:06Z

dc.date.available

2023-10-22T01:17:06Z

dc.date.issued

2020-02

dc.date.updated

2023-10-22T01:17:04Z

dc.description.abstract

Hypothesis

Microneedles can create microperforations in the round window membrane (RWM) without causing anatomic or physiologic damage.

Background

Reliable delivery of agents into the inner ear for therapeutic and diagnostic purposes remains a challenge. Our novel approach employs microneedles to facilitate intracochlear access via the RWM. This study investigates the anatomical and functional consequences of microneedle perforations in guinea pig RWMs in vivo.

Methods

Single three-dimensional-printed, 100 μm diameter microneedles were used to perforate the guinea pig RWM via the postauricular sulcus. Hearing was assessed both before and after microneedle perforation using compound action potential and distortion product otoacoustic emissions. Confocal microscopy was used ex vivo to examine harvested RWMs, measuring the size, shape, and location of perforations and documenting healing at 0 hours (n = 7), 24 hours (n = 6), 48 hours (n = 6), and 1 week (n = 6).

Results

Microneedles create precise and accurate perforations measuring 93.1 ± 29.0 μm by 34.5 ± 16.8 μm and produce a high-frequency threshold shift that disappears after 24 hours. Examination of perforations over time demonstrates healing progression over 24 to 48 hours and complete perforation closure by 1 week.

Conclusion

Microneedles can create a temporary microperforation in the RWM without causing significant anatomic or physiologic dysfunction. Microneedles have the potential to mediate safe and effective intracochlear access for diagnosis and treatment of inner ear disease.
dc.identifier

00129492-202002000-00040

dc.identifier.issn

1531-7129

dc.identifier.issn

1537-4505

dc.identifier.uri

https://hdl.handle.net/10161/29299

dc.language

eng

dc.publisher

Ovid Technologies (Wolters Kluwer Health)

dc.relation.ispartof

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology

dc.relation.isversionof

10.1097/mao.0000000000002491

dc.subject

Animals

dc.subject

Guinea Pigs

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Needles

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Hearing

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Ear, Inner

dc.subject

Round Window, Ear

dc.title

Anatomical and Functional Consequences of Microneedle Perforation of Round Window Membrane.

dc.type

Journal article

duke.contributor.orcid

Chiang, Harry|0000-0003-1693-5110

pubs.begin-page

e280

pubs.end-page

e287

pubs.issue

2

pubs.organisational-group

Duke

pubs.organisational-group

School of Medicine

pubs.organisational-group

Staff

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Surgery

pubs.organisational-group

Head and Neck Surgery & Communication Sciences

pubs.publication-status

Published

pubs.volume

41

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