Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs.

Okazaki, Y

Furuno, M

Kasukawa, T

Adachi, J

Bono, H

Kondo, S

Nikaido, I

Osato, N

Osato, N

Saito, R

Suzuki, H

Yamanaka, I

Kiyosawa, H

Yagi, K

Tomaru, Y

Hasegawa, Y

Nogami, A

Schönbach, C

Gojobori, T

Baldarelli, R

Hill, DP

Bult, C

Hume, DA

Hume, DA

Quackenbush, J

Schriml, LM

Kanapin, A

Matsuda, H

Batalov, S

Beisel, KW

Blake, JA

Bradt, D

Brusic, V

Chothia, C

Corbani, LE

Cousins, S

Dalla, E

Dragani, TA

Fletcher, CF

Forrest, A

Frazer, KS

Gaasterland, T

Gariboldi, M

Gissi, C

Godzik, A

Gough, J

Grimmond, S

Gustincich, S

Hirokawa, N

Jackson, IJ

Jarvis, ED

Kanai, A

Kawaji, H

Kawasawa, Y

Kedzierski, RM

King, BL

Konagaya, A

Kurochkin, IV

Lee, Y

Lenhard, B

Lyons, PA

Maglott, DR

Maltais, L

Marchionni, L

McKenzie, L

Miki, H

Nagashima, T

Numata, K

Okido, T

Pavan, WJ

Pertea, G

Pesole, G

Petrovsky, N

Pillai, R

Pontius, JU

Qi, D

Ramachandran, S

Ravasi, T

Reed, JC

Reed, DJ

Reid, J

Ring, BZ

Ringwald, M

Sandelin, A

Schneider, C

Semple, CAM

Setou, M

Shimada, K

Sultana, R

Takenaka, Y

Taylor, MS

Teasdale, RD

Tomita, M

Verardo, R

Wagner, L

Wahlestedt, C

Wang, Y

Watanabe, Y

Wells, C

Wilming, LG

Wynshaw-Boris, A

Yanagisawa, M

Yang, I

Yang, L

Yuan, Z

Zavolan, M

Zhu, Y

Zimmer, A

Carninci, P

Hayatsu, N

Hirozane-Kishikawa, T

Konno, H

Nakamura, M

Sakazume, N

Sato, K

Shiraki, T

Waki, K

Kawai, J

Aizawa, K

Arakawa, T

Fukuda, S

Hara, A

Hashizume, W

Imotani, K

Ishii, Y

Itoh, M

Kagawa, I

Miyazaki, A

Sakai, K

Sasaki, D

Shibata, K

Shinagawa, A

Yasunishi, A

Yoshino, M

Waterston, R

Lander, ES

Rogers, J

Birney, E

Hayashizaki, Y

FANTOM Consortium

RIKEN Genome Exploration Research Group Phase I & II Team

England

2015-12-19T14:09:11Z

2002-12-05

Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics.

https://www.ncbi.nlm.nih.gov/pubmed/12466851

nature01266

0028-0836

https://hdl.handle.net/10161/11223

eng

Springer Science and Business Media LLC

Nature

10.1038/nature01266

Alternative Splicing

Amino Acid Motifs

Animals

Chromosomes, Mammalian

Cloning, Molecular

DNA, Complementary

Databases, Genetic

Expressed Sequence Tags

Genes

Genomics

Humans

Membrane Proteins

Mice

Physical Chromosome Mapping

Protein Structure, Tertiary

Proteome

RNA, Antisense

RNA, Messenger

RNA, Untranslated

Transcription Initiation Site

Transcription, Genetic

Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs.

Journal article

https://www.ncbi.nlm.nih.gov/pubmed/12466851

563

573

6915

Basic Science Departments

Duke

Duke Institute for Brain Sciences

Institutes and Provost's Academic Units

Neurobiology

School of Medicine

University Institutes and Centers

Published

420