Imaging Polarization in Budding Yeast.
| dc.contributor.author | McClure, Allison W | |
| dc.contributor.author | Wu, Chi-Fang | |
| dc.contributor.author | Johnson, Sam A | |
| dc.contributor.author | Lew, Daniel J | |
| dc.coverage.spatial | United States | |
| dc.date.accessioned | 2016-12-01T14:21:33Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected aspects of polarization including transient intermediates with more than one polarity cluster, oscillatory clustering of polarity factors, and mobile "wandering" polarity sites. | |
| dc.identifier | ||
| dc.identifier.eissn | 1940-6029 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Springer New York | |
| dc.relation.ispartof | Methods Mol Biol | |
| dc.relation.isversionof | 10.1007/978-1-4939-3480-5_2 | |
| dc.subject | Cdc42 | |
| dc.subject | Cell polarity | |
| dc.subject | GFP | |
| dc.subject | Imaging | |
| dc.subject | Microscopy | |
| dc.subject | Polarity establishment | |
| dc.subject | Yeast | |
| dc.title | Imaging Polarization in Budding Yeast. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Lew, Daniel J|0000-0001-7482-3585 | |
| pubs.author-url | ||
| pubs.begin-page | 13 | |
| pubs.end-page | 23 | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Cell Biology | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Molecular Genetics and Microbiology | |
| pubs.organisational-group | Pharmacology & Cancer Biology | |
| pubs.organisational-group | School of Medicine | |
| pubs.publication-status | Published | |
| pubs.volume | 1407 |
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