Intravital imaging of mouse embryos
dc.contributor.author | Huang, Qiang | |
dc.contributor.author | Cohen, Malkiel A | |
dc.contributor.author | Alsina, Fernando C | |
dc.contributor.author | Devlin, Garth | |
dc.contributor.author | Garrett, Aliesha | |
dc.contributor.author | McKey, Jennifer | |
dc.contributor.author | Havlik, Patrick | |
dc.contributor.author | Rakhilin, Nikolai | |
dc.contributor.author | Wang, Ergang | |
dc.contributor.author | Xiang, Kun | |
dc.contributor.author | Mathews, Parker | |
dc.contributor.author | Wang, Lihua | |
dc.contributor.author | Bock, Cheryl | |
dc.contributor.author | Ruthig, Victor | |
dc.contributor.author | Wang, Yi | |
dc.contributor.author | Negrete, Marcos | |
dc.contributor.author | Wong, Chi Wut | |
dc.contributor.author | Murthy, Preetish KL | |
dc.contributor.author | Zhang, Shupei | |
dc.contributor.author | Daniel, Andrea R | |
dc.contributor.author | Kirsch, David G | |
dc.contributor.author | Kang, Yubin | |
dc.contributor.author | Capel, Blanche | |
dc.contributor.author | Asokan, Aravind | |
dc.contributor.author | Silver, Debra L | |
dc.contributor.author | Jaenisch, Rudolf | |
dc.contributor.author | Shen, Xiling | |
dc.date.accessioned | 2020-04-11T04:22:33Z | |
dc.date.available | 2020-04-11T04:22:33Z | |
dc.date.issued | 2020-04-10 | |
dc.date.updated | 2020-04-11T04:22:32Z | |
dc.description.abstract | <jats:p>Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)–derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.</jats:p> | |
dc.identifier.issn | 0036-8075 | |
dc.identifier.issn | 1095-9203 | |
dc.identifier.uri | ||
dc.language | en | |
dc.publisher | American Association for the Advancement of Science (AAAS) | |
dc.relation.ispartof | Science | |
dc.relation.isversionof | 10.1126/science.aba0210 | |
dc.title | Intravital imaging of mouse embryos | |
dc.type | Journal article | |
duke.contributor.orcid | Alsina, Fernando C|0000-0002-6304-7688 | |
duke.contributor.orcid | Wong, Chi Wut|0000-0002-1703-785X | |
duke.contributor.orcid | Capel, Blanche|0000-0002-6587-0969 | |
duke.contributor.orcid | Asokan, Aravind|0000-0001-5563-4877 | |
duke.contributor.orcid | Silver, Debra L|0000-0001-9189-844X | |
duke.contributor.orcid | Shen, Xiling|0000-0002-4978-3531 | |
pubs.begin-page | 181 | |
pubs.end-page | 186 | |
pubs.issue | 6487 | |
pubs.organisational-group | Pratt School of Engineering | |
pubs.organisational-group | Biomedical Engineering | |
pubs.organisational-group | Electrical and Computer Engineering | |
pubs.organisational-group | Duke Cancer Institute | |
pubs.organisational-group | Molecular Genetics and Microbiology | |
pubs.organisational-group | Duke | |
pubs.organisational-group | Institutes and Centers | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | Basic Science Departments | |
pubs.organisational-group | Cell Biology | |
pubs.organisational-group | Neurobiology | |
pubs.organisational-group | Duke Science & Society | |
pubs.organisational-group | Duke Institute for Brain Sciences | |
pubs.organisational-group | Initiatives | |
pubs.organisational-group | Institutes and Provost's Academic Units | |
pubs.organisational-group | University Institutes and Centers | |
pubs.organisational-group | Student | |
pubs.organisational-group | Pharmacology & Cancer Biology | |
pubs.organisational-group | Surgery, Surgical Sciences | |
pubs.organisational-group | Surgery | |
pubs.organisational-group | Clinical Science Departments | |
pubs.publication-status | Published | |
pubs.volume | 368 |
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