Intravital imaging of mouse embryos

dc.contributor.author

Huang, Qiang

dc.contributor.author

Cohen, Malkiel A

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Alsina, Fernando C

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Devlin, Garth

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Garrett, Aliesha

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McKey, Jennifer

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Havlik, Patrick

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Rakhilin, Nikolai

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Wang, Ergang

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Xiang, Kun

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Mathews, Parker

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Wang, Lihua

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Bock, Cheryl

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Ruthig, Victor

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Wang, Yi

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Negrete, Marcos

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Wong, Chi Wut

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Murthy, Preetish KL

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Zhang, Shupei

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Daniel, Andrea R

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Kirsch, David G

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Kang, Yubin

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Capel, Blanche

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Asokan, Aravind

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Silver, Debra L

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Jaenisch, Rudolf

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Shen, Xiling

dc.date.accessioned

2020-04-11T04:22:33Z

dc.date.available

2020-04-11T04:22:33Z

dc.date.issued

2020-04-10

dc.date.updated

2020-04-11T04:22:32Z

dc.description.abstract

<jats:p>Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)–derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.</jats:p>

dc.identifier.issn

0036-8075

dc.identifier.issn

1095-9203

dc.identifier.uri

https://hdl.handle.net/10161/20403

dc.language

en

dc.publisher

American Association for the Advancement of Science (AAAS)

dc.relation.ispartof

Science

dc.relation.isversionof

10.1126/science.aba0210

dc.title

Intravital imaging of mouse embryos

dc.type

Journal article

duke.contributor.orcid

Alsina, Fernando C|0000-0002-6304-7688

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Wong, Chi Wut|0000-0002-1703-785X

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Capel, Blanche|0000-0002-6587-0969

duke.contributor.orcid

Asokan, Aravind|0000-0001-5563-4877

duke.contributor.orcid

Silver, Debra L|0000-0001-9189-844X

duke.contributor.orcid

Shen, Xiling|0000-0002-4978-3531

pubs.begin-page

181

pubs.end-page

186

pubs.issue

6487

pubs.organisational-group

Pratt School of Engineering

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Biomedical Engineering

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Electrical and Computer Engineering

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Duke Cancer Institute

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Molecular Genetics and Microbiology

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Duke

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Institutes and Centers

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School of Medicine

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Basic Science Departments

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Cell Biology

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Neurobiology

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Duke Science & Society

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Duke Institute for Brain Sciences

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Initiatives

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Institutes and Provost's Academic Units

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University Institutes and Centers

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Student

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Pharmacology & Cancer Biology

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Surgery, Surgical Sciences

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Surgery

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Clinical Science Departments

pubs.publication-status

Published

pubs.volume

368

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