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The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.
Abstract
Transgenic labeling of innate immune cell lineages within the larval zebrafish allows
for real-time, in vivo analyses of microbial pathogenesis within a vertebrate host.
To date, labeling of zebrafish macrophages has been relatively limited, with the most
specific expression coming from the mpeg1 promoter. However, mpeg1 transcription at
both endogenous and transgenic loci becomes attenuated in the presence of intracellular
pathogens, including Salmonella typhimurium and Mycobacterium marinum. Here, we describe
mfap4 as a macrophage-specific promoter capable of producing transgenic lines in which
transgene expression within larval macrophages remains stable throughout several days
of infection. Additionally, we have developed a novel macrophage-specific Cre transgenic
line under the control of mfap4, enabling macrophage-specific expression using existing
floxed transgenic lines. These tools enrich the repertoire of transgenic lines and
promoters available for studying zebrafish macrophage dynamics during infection and
inflammation and add flexibility to the design of future macrophage-specific transgenic
lines.
Type
Journal articleSubject
AnimalsAnimals, Genetically Modified
Cell Lineage
Disease Models, Animal
Host-Pathogen Interactions
Immunity, Innate
Larva
Macrophages
Mycobacterium Infections
Mycobacterium marinum
Promoter Regions, Genetic
Salmonella typhimurium
Transgenes
Zebrafish
Zebrafish Proteins
Permalink
https://hdl.handle.net/10161/10845Published Version (Please cite this version)
10.1371/journal.pone.0138949Publication Info
Walton, Eric M; Cronan, Mark R; Beerman, Rebecca W; & Tobin, David M (2015). The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage
Behavior during Mycobacterial Infection in Zebrafish. PLoS One, 10(10). pp. e0138949. 10.1371/journal.pone.0138949. Retrieved from https://hdl.handle.net/10161/10845.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Rebecca Beerman
Program Mgr, Clinical Data Mgmt
David M. Tobin
Professor of Molecular Genetics and Microbiology
Tuberculosis: Mycobacterial Pathogenesis and Host Susceptibility
Tuberculosis kills 1.5 million people annually. Our laboratory aims to understand
the intricate interplay between mycobacteria and their hosts using a combination of
model organism genetics, human genetics, pharmacology and high-resolution microscopy.
By identifying key pathways utilized by the infecting bacteria and the host innate
immune system, we hope to discover new therapeutic targets and interventi
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