Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.
Abstract
Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play
an important role in the development of organs-on-a-chip. Most TEBV construction involves
scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. Hypothesizing
that a scaffold-free TEBV may be advantageous, we constructed a tubular structure
(1 mm i.d.) from aligned human mesenchymal cell sheets (hMSC) as the wall and human
endothelial progenitor cell (hEPC) coating as the lumen. The burst pressure of the
scaffold-free TEBV was above 200 mmHg after three weeks of sequential culture in a
rotating wall bioreactor and perfusion at 6.8 dynes/cm(2). The interwoven organization
of the cell layers and extensive extracellular matrix (ECM) formation of the hMSC-based
TEBV resembled that of native blood vessels. The TEBV exhibited flow-mediated vasodilation,
vasoconstriction after exposure to 1 μM phenylephrine and released nitric oxide in
a manner similar to that of porcine femoral vein. HL-60 cells attached to the TEBV
lumen after TNF-α activation to suggest a functional endothelium. This study demonstrates
the potential of a hEPC endothelialized hMSC-based TEBV for drug screening.
Type
Journal articleSubject
AnimalsBioreactors
Blood Vessel Prosthesis
Cell Adhesion
Cell Culture Techniques
Extracellular Matrix
Femoral Vein
HL-60 Cells
Human Umbilical Vein Endothelial Cells
Humans
Mesenchymal Stromal Cells
Nitric Oxide
Perfusion
Phenylephrine
Pressure
Swine
Tissue Engineering
Tumor Necrosis Factor-alpha
Vasoconstriction
Vasodilation
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https://hdl.handle.net/10161/12401Published Version (Please cite this version)
10.1038/srep15116Publication Info
Jung, Y; Ji, H; Chen, Z; Fai Chan, H; Atchison, L; Klitzman, B; ... Leong, KW (2015). Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.
Sci Rep, 5. pp. 15116. 10.1038/srep15116. Retrieved from https://hdl.handle.net/10161/12401.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Bruce Klitzman
Associate Professor Emeritus in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors,
and long term improvement of medical device implantation. My basic research interests
are in the area of physiological mechanisms of optimizing substrate transport to tissue.
This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular,
cellular, ultrastructural, and molecular level. The current projects include:
1) control of blood flow and flow distribu
Kam Leong
Adjunct Professor of Biomedical Engineering
Professor Leong's research interest focuses on biomaterials design, particularly on
synthesis of nanoparticles for DNA-based therapeutics, and nanostructured biomaterials
for regenerative medicine
Biomaterials Design:
design of self-assembled fibers for tissue engineering
microfluidics-mediated synthesis of multifunctional nanoparticles for drug and gene
delivery
synthesis of novel quantum dots for biomedical applications
Con
This author no longer has a Scholars@Duke profile, so the information shown here reflects
their Duke status at the time this item was deposited.
George A. Truskey
R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering
My research interests focus upon the effect of physical forces on the function of
vascular cells and skeletal muscle, cell adhesion, and the design of engineered tissues.
Current research projects examine the effect of endothelial cell senescence upon
permeability to macromolecules and the response to fluid shear stress, the development
of microphysiological blood vessels and muscles for evaluation of drug toxicity and
the design of engineered endothelialized blood vessels and skelet
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