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The cell division protein MinD from Pseudomonas aeruginosa dominates the assembly of the MinC-MinD copolymers.

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Date
2018-04-02
Authors
Huang, Haiyan
Wang, Ping
Bian, Li
Osawa, Masaki
Erickson, Harold P
Chen, Yaodong
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Abstract
Cell division of rod-shaped bacteria requires the Z ring, a ring of FtsZ filaments associated with the inner-membrane wall. The MinCDE proteins help localize the Z ring to the center of the Escherichia coli cell. MinC, which inhibits Z-ring assembly, is a passenger on MinD. Previous studies have shown that MinC-MinD from E. coli and Aquifex aeolicus assemble in vitro into extended filaments with a 1:1 stoichiometry. However, a recent study has raised questions about the function of the MinC-MinD copolymer in vivo, since its assembly appears to require a high concentration of these two proteins, has a long lag time, and its blockade does not affect in vivo activities. Here, we found that MinC and MinD from Pseudomonas aeruginosa coassemble into filaments with a 1:1 stoichiometry. We also found that the minimal concentration of ~4 μM required for assembly applies only to MinD because above 4 μM MinD, even very low MinC concentrations sustained coassembly. As previously reported, the MinC-MinD coassembly exhibited a long lag of ~100 s when initiated by ATP. Premixing MinD with ATP eliminated this lag, suggesting that it may be due to slow MinD dimerization following ATP activation. We also discovered that MinC-MinD copolymers quickly bound and formed huge bundles with FtsZ filaments. Our results resolve previous questions about the low concentration of MinC and the lag time, insights that may inform future investigations into the exact role of the MinC-MinD copolymer in vivo.
Type
Journal article
Subject
FtsZ
MinC-MinD copolymer
MinCDE proteins
Z-ring
bacteria
cell division
cytoskeleton
electron microscopy (EM)
min system
protein dynamic
Permalink
https://hdl.handle.net/10161/16623
Published Version (Please cite this version)
10.1074/jbc.ra117.001513
Publication Info
Huang, Haiyan; Wang, Ping; Bian, Li; Osawa, Masaki; Erickson, Harold P; & Chen, Yaodong (2018). The cell division protein MinD from Pseudomonas aeruginosa dominates the assembly of the MinC-MinD copolymers. The Journal of biological chemistry. 10.1074/jbc.ra117.001513. Retrieved from https://hdl.handle.net/10161/16623.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Erickson

Harold Paul Erickson

James B. Duke Distinguished Professor of Cell Biology
Cytoskeleton: It is now clear that the actin and microtubule cytoskeleton originated in bacteria. Our major research is on FtsZ, the bacterial tubulin homolog, which assembles into a contractile ring that divides the bacterium. We have studied FtsZ assembly in vitro, and found that it assembles into thin protofilaments (pfs). Dozens of these pfs are further clustered to form the contractile Z-ring in vivo. Some important discoveries in the last ten years include: &bul

Masaki Osawa

Assistant Research Professor of Cell Biology
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