Intravital imaging of mouse embryos
Abstract
<jats:p>Embryonic development is a complex process that is unamenable to direct observation.
In this study, we implanted a window to the mouse uterus to visualize the developing
embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation
and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission
and early vascularization of neural crest cell (NCC)–derived perivascular cells in
the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through
the placenta. We combined the imaging window with in utero electroporation to label
and track cell division and movement within embryos and observed that clusters of
mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human
donor NCC-derived cells shrank. This technique can be combined with various tissue
manipulation and microscopy methods to study the processes of development at unprecedented
spatiotemporal resolution.</jats:p>
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Journal articlePermalink
https://hdl.handle.net/10161/20403Published Version (Please cite this version)
10.1126/science.aba0210Publication Info
(2020). Intravital imaging of mouse embryos. Science, 368(6487). pp. 181-186. 10.1126/science.aba0210. Retrieved from https://hdl.handle.net/10161/20403.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Fernando Alsina
Research Scholar
I am from Argentina and I did my graduate training in Dr. Gustavo Paratcha´s
lab in the Institute of Cellular Biology and Neuroscience “Prof. E. De Robertis”
(IBCN) in the University of Buenos Aires (UBA – CONICET), Argentina. My PhD
thesis focused on the negative regulation of neurotrophic factor receptor signaling
in developing neurons.Personal Interests: In my free time I really enjoy hiking, playing
soccer, going out with friends and family, traveling around, re
Blanche Capel
James B. Duke Distinguished Professor of Cell Biology
In mammals, the primary step in male sex determination is the initiation of testis
development in the bipotential gonad primordium. This step depends on the Y-linked
male sex-determining gene, Sry. Expression of Sry in the XY gonad, or as a transgene
in an XX gonad, leads to the differentiation of Sertoli cells. Failures in Sertoli
cell differentiation in the XY gonad result in sex reversal and ovary formation. In
addition to Sertoli cell differentiation, we are studying the s
Yubin Kang
Professor of Medicine
David Guy Kirsch
Barbara Levine University Distinguished Professor
My clinical interests are the multi-modality care of patients with bone and soft tissue
sarcomas and developing new sarcoma therapies. My laboratory interests include utilizing
mouse models of cancer to study cancer and radiation biology in order to develop new
cancer therapies in the pre-clinical setting.
Xiling Shen
Hawkins Family Associate Professor
Dr. Shen’s research interests lie at precision medicine and systems biology. His lab
integrates engineering, computational and biological techniques to study cancer, stem
cells, microbiota and the nervous system in the gut. This multidisciplinary work has
been instrumental in initiating several translational clinical trials in precision
therapy. He is the director of the Woo Center for Big Data and Precision Health (DAP)
and a core member of the Center for Genomics and Computational Biolog
This author no longer has a Scholars@Duke profile, so the information shown here reflects
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Debra Lynn Silver
Associate Professor of Molecular Genetics and Microbiology
How is the brain assembled and sculpted during embryonic development? Addressing
this question has enormous implications for understanding neurodevelopmental disorders
affecting brain size and function. In evolutionary terms, our newest brain structure
is the cerebral cortex, which drives higher cognitive capacities. The overall mission
of my research lab is to elucidate genetic and cellular mechanisms controlling cortical
development and contributing to neurodevelopmental patho
Chi Wut Wong
Student
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