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Intravital imaging of mouse embryos

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Date
2020-04-10
Authors
Huang, Qiang
Cohen, Malkiel A
Alsina, Fernando C
Devlin, Garth
Garrett, Aliesha
McKey, Jennifer
Havlik, Patrick
Rakhilin, Nikolai
Wang, Ergang
Xiang, Kun
Mathews, Parker
Wang, Lihua
Bock, Cheryl
Ruthig, Victor
Wang, Yi
Negrete, Marcos
Wong, Chi Wut
Murthy, Preetish KL
Zhang, Shupei
Daniel, Andrea R
Kirsch, David G
Kang, Yubin
Capel, Blanche
Asokan, Aravind
Silver, Debra L
Jaenisch, Rudolf
Shen, Xiling
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(27 total)
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Abstract
<jats:p>Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)–derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.</jats:p>
Type
Journal article
Permalink
https://hdl.handle.net/10161/20403
Published Version (Please cite this version)
10.1126/science.aba0210
Publication Info
Huang, Qiang; Cohen, Malkiel A; Alsina, Fernando C; Devlin, Garth; Garrett, Aliesha; McKey, Jennifer; ... Shen, Xiling (2020). Intravital imaging of mouse embryos. Science, 368(6487). pp. 181-186. 10.1126/science.aba0210. Retrieved from https://hdl.handle.net/10161/20403.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Alsina

Fernando Alsina

Research Scholar
I am from Argentina and I did my graduate training in Dr. Gustavo Paratcha&acute;s lab in the Institute of Cellular Biology and Neuroscience &ldquo;Prof. E. De Robertis&rdquo; (IBCN) in the University of Buenos Aires (UBA &ndash; CONICET), Argentina. My PhD thesis focused on the negative regulation of neurotrophic factor receptor signaling in developing neurons.Personal Interests: In my free time I really enjoy hiking, playing soccer, going out with friends and family, traveling around, re
Asokan

Aravind Asokan

Professor in Surgery
Synthetic Virology & Gene Therapy
Capel

Blanche Capel

James B. Duke Distinguished Professor of Cell Biology
In mammals, the primary step in male sex determination is the initiation of testis development in the bipotential gonad primordium. This step depends on the Y-linked male sex-determining gene, Sry. Expression of Sry in the XY gonad, or as a transgene in an XX gonad, leads to the differentiation of Sertoli cells. Failures in Sertoli cell differentiation in the XY gonad result in sex reversal and ovary formation. In addition to Sertoli cell differentiation, we are studying the s
Kang

Yubin Kang

Professor of Medicine
Kirsch

David Guy Kirsch

Barbara Levine University Distinguished Professor
My clinical interests are the multi-modality care of patients with bone and soft tissue sarcomas and developing new sarcoma therapies. My laboratory interests include utilizing mouse models of cancer to study cancer and radiation biology in order to develop new cancer therapies in the pre-clinical setting.
Shen

Xiling Shen

Hawkins Family Associate Professor
Dr. Shen’s research interests lie at precision medicine and systems biology. His lab integrates engineering, computational and biological techniques to study cancer, stem cells, microbiota and the nervous system in the gut. This multidisciplinary work has been instrumental in initiating several translational clinical trials in precision therapy. He is the director of the Woo Center for Big Data and Precision Health (DAP) and a core member of the Center for Genomics and Computational Biolog
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.
Silver

Debra Lynn Silver

Associate Professor of Molecular Genetics and Microbiology
How is the brain assembled and sculpted during embryonic development?  Addressing this question has enormous implications for understanding neurodevelopmental disorders affecting brain size and function. In evolutionary terms, our newest brain structure is the cerebral cortex, which drives higher cognitive capacities. The overall mission of my research lab is to elucidate genetic and cellular mechanisms controlling cortical development and contributing to neurodevelopmental patho

Chi Wut Wong

Student
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