Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice.
Abstract
<h4>Background and objective</h4>IPF is a fatal and debilitating lung disorder increasing
in incidence worldwide. To date, two approved treatments only slow disease progression,
have multiple side effects and do not provide a cure. MSC have promising therapeutic
potential as a cell-based therapy for many lung disorders based on the anti-fibrotic
properties of the MSC.<h4>Methods</h4>Critical questions remain surrounding the optimal
source, timing and efficacy of cell-based therapies. The present study examines the
most effective sources of MSC. Human MSC were derived from adipose, WJ, chorionic
membrane (CSC) and chorionic villi (CVC). MSC were injected into the ageing mouse
model of BLM-induced lung fibrosis.<h4>Results</h4>All sources decreased Aschroft
and hydroxyproline levels when injected into BLM-treated mice at day 10 with the exception
of CSC cells that did not change hydroxyproline levels. There were also decreases
in mRNA expression of αv -integrin and TNFα in all sources except CSC. Only ASC- and WJ-derived cells reduced
AKT and MMP-2 activation, while Cav-1 was increased by ASC treatment as previously
reported. BLM-induced miR dysregulation of miR-29 and miR-199 was restored only by
ASC treatment.<h4>Conclusion</h4>Our data suggest that sources of MSC may differ in
the pathway(s) involved in repair.
Type
Journal articleSubject
Mesenchymal Stem CellsAnimals
Mice, Inbred C57BL
Humans
Pulmonary Fibrosis
Disease Models, Animal
Inflammation
Bleomycin
MicroRNAs
RNA, Messenger
Mesenchymal Stem Cell Transplantation
Transplantation, Homologous
Gene Expression Regulation
Adult
Male
Proto-Oncogene Proteins c-akt
Caveolin 1
Matrix Metalloproteinase 2
Biomarkers
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https://hdl.handle.net/10161/24724Published Version (Please cite this version)
10.1111/resp.13928Publication Info
Periera-Simon, Simone; Xia, Xiaomei; Catanuto, Paola; Coronado, Ramon; Kurtzberg,
Joanne; Bellio, Michael; ... Glassberg, Marilyn K (2021). Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis
in C57BL6 male mice. Respirology (Carlton, Vic.), 26(2). pp. 161-170. 10.1111/resp.13928. Retrieved from https://hdl.handle.net/10161/24724.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Joanne Kurtzberg
Jerome S. Harris Distinguished Professor of Pediatrics
Dr. Kurtzberg is an internationally renowned expert in pediatric hematology/oncology,
pediatric blood and marrow transplantation, umbilical cord blood banking and transplantation,
and novel applications of cord blood and birthing tissues in the emerging fields of
cellular therapies and regenerative medicine. Dr. Kurtzberg serves as the Director
of the Marcus Center for Cellular Cures (MC3), Director of the Pediatric Transplant
and Cellular Therapy Program, Director of the Carolina

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