Noninvasive monitoring of tissue hemoglobin using UV-VIS diffuse reflectance spectroscopy: a pilot study.
Abstract
We conducted a pilot study on 10 patients undergoing general surgery to test the feasibility
of diffuse reflectance spectroscopy in the visible wavelength range as a noninvasive
monitoring tool for blood loss during surgery. Ratios of raw diffuse reflectance at
wavelength pairs were tested as a first-pass for estimating hemoglobin concentration.
Ratios can be calculated easily and rapidly with limited post-processing, and so this
can be considered a near real-time monitoring device. We found the best hemoglobin
correlations were when ratios at isosbestic points of oxy- and deoxyhemoglobin were
used, specifically 529/500 nm. Baseline subtraction improved correlations, specifically
at 520/509 nm. These results demonstrate proof-of-concept for the ability of this
noninvasive device to monitor hemoglobin concentration changes due to surgical blood
loss. The 529/500 nm ratio also appears to account for variations in probe pressure,
as determined from measurements on two volunteers.
Type
Journal articleSubject
BiomarkersBlood Chemical Analysis
Blood Loss, Surgical
Diagnosis, Computer-Assisted
Feasibility Studies
Hemoglobins
Humans
Oxygen
Pilot Projects
Postoperative Hemorrhage
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
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Show full item recordScholars@Duke
Eugene William Moretti
Professor of Anesthesiology
Research efforts are focused primarily in the area of functional genomics. Work has
centered on investigating genetic polymorphisms in the surgical intensive care population
that would predispose one to the development of the sepsis syndrome. As an extension
of this work, there is ongoing investigation working to identify genetically susceptible
populations at risk for developing various types of perioperative organ dysfunction.
Parallel studies involve identification of a panel of biomark

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