High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection.
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We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.
Genetic Predisposition to Disease
Polymorphism, Single Nucleotide
Published Version (Please cite this version)10.1371/journal.pone.0002882
Publication InfoFuhrman, Laura E; Shianna, Kevin V; & Aballay, Alejandro (2008). High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection. PLoS One, 3(8). pp. e2882. 10.1371/journal.pone.0002882. Retrieved from https://hdl.handle.net/10161/4499.
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Adjunct Professor in the Department of Molecular Genetics and Microbiology
Our laboratory uses genetic and functional genomic methodologies to study the genetic basis of innate immunity using C. elegans and mammalian systems. Recent studies from our laboratory highlight the importance of the nervous system in the regulation of innate immune responses. Using a genetic approach we were able to demonstrate that specific neurons can regulate innate immunit