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Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration.

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Date
2013-10
Authors
Francisco, Aubrey T
Mancino, Robert J
Bowles, Robby D
Brunger, Jonathan M
Tainter, David M
Chen, Yi-Te
Richardson, William J
Guilak, Farshid
Setton, Lori A
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Abstract
Cell delivery to the pathological intervertebral disc (IVD) has significant therapeutic potential for enhancing IVD regeneration. The development of injectable biomaterials that retain delivered cells, promote cell survival, and maintain or promote an NP cell phenotype in vivo remains a significant challenge. Previous studies have demonstrated NP cell - laminin interactions in the nucleus pulposus (NP) region of the IVD that promote cell attachment and biosynthesis. These findings suggest that incorporating laminin ligands into carriers for cell delivery may be beneficial for promoting NP cell survival and phenotype. Here, an injectable, laminin-111 functionalized poly(ethylene glycol) (PEG-LM111) hydrogel was developed as a biomaterial carrier for cell delivery to the IVD. We evaluated the mechanical properties of the PEG-LM111 hydrogel, and its ability to retain delivered cells in the IVD space. Gelation occurred in approximately 20 min without an initiator, with dynamic shear moduli in the range of 0.9-1.4 kPa. Primary NP cell retention in cultured IVD explants was significantly higher over 14 days when cells were delivered within a PEG-LM111 carrier, as compared to cells in liquid suspension. Together, these results suggest this injectable laminin-functionalized biomaterial may be an easy to use carrier for delivering cells to the IVD.
Type
Journal article
Subject
Injectable
Intervertebral disc
Laminin
Nucleus pulposus
Organ culture
Polyethylene glycol
Animals
Biocompatible Materials
Cells, Cultured
Humans
Hydrogel
Injections
Intervertebral Disc
Laminin
Luciferases
Mechanical Phenomena
Polyethylene Glycols
Rats
Rats, Sprague-Dawley
Regeneration
Rheology
Sus scrofa
Permalink
https://hdl.handle.net/10161/7858
Published Version (Please cite this version)
10.1016/j.biomaterials.2013.06.038
Publication Info
Francisco, Aubrey T; Mancino, Robert J; Bowles, Robby D; Brunger, Jonathan M; Tainter, David M; Chen, Yi-Te; ... Setton, Lori A (2013). Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration. Biomaterials, 34(30). pp. 7381-7388. 10.1016/j.biomaterials.2013.06.038. Retrieved from https://hdl.handle.net/10161/7858.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Farshid Guilak

Lazlo Ormandy Professor of Orthopaedic Surgery
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.

Lori A. Setton

Adjunct Professor of Biomedical Engineering
Research in Setton's laboratory is focused on the role of mechanical factors in the degeneration and repair of soft tissues of the musculoskeletal system, including the intervertebral disc, articular cartilage and meniscus. Work in the Laboratory is focused on engineering and evaluating materials for tissue regeneration and drug delivery. Studies combining engineering and biology are also used to determine the role of mechanical factors to promote and control healing of cartilaginous tissues. Re
Alphabetical list of authors with Scholars@Duke profiles.
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