Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration.
Abstract
Cell delivery to the pathological intervertebral disc (IVD) has significant therapeutic
potential for enhancing IVD regeneration. The development of injectable biomaterials
that retain delivered cells, promote cell survival, and maintain or promote an NP
cell phenotype in vivo remains a significant challenge. Previous studies have demonstrated
NP cell - laminin interactions in the nucleus pulposus (NP) region of the IVD that
promote cell attachment and biosynthesis. These findings suggest that incorporating
laminin ligands into carriers for cell delivery may be beneficial for promoting NP
cell survival and phenotype. Here, an injectable, laminin-111 functionalized poly(ethylene
glycol) (PEG-LM111) hydrogel was developed as a biomaterial carrier for cell delivery
to the IVD. We evaluated the mechanical properties of the PEG-LM111 hydrogel, and
its ability to retain delivered cells in the IVD space. Gelation occurred in approximately
20 min without an initiator, with dynamic shear moduli in the range of 0.9-1.4 kPa.
Primary NP cell retention in cultured IVD explants was significantly higher over 14
days when cells were delivered within a PEG-LM111 carrier, as compared to cells in
liquid suspension. Together, these results suggest this injectable laminin-functionalized
biomaterial may be an easy to use carrier for delivering cells to the IVD.
Type
Journal articleSubject
InjectableIntervertebral disc
Laminin
Nucleus pulposus
Organ culture
Polyethylene glycol
Animals
Biocompatible Materials
Cells, Cultured
Humans
Hydrogel
Injections
Intervertebral Disc
Laminin
Luciferases
Mechanical Phenomena
Polyethylene Glycols
Rats
Rats, Sprague-Dawley
Regeneration
Rheology
Sus scrofa
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https://hdl.handle.net/10161/7858Published Version (Please cite this version)
10.1016/j.biomaterials.2013.06.038Publication Info
Francisco, Aubrey T; Mancino, Robert J; Bowles, Robby D; Brunger, Jonathan M; Tainter,
David M; Chen, Yi-Te; ... Setton, Lori A (2013). Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration. Biomaterials, 34(30). pp. 7381-7388. 10.1016/j.biomaterials.2013.06.038. Retrieved from https://hdl.handle.net/10161/7858.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Farshid Guilak
Lazlo Ormandy Professor of Orthopaedic Surgery
This author no longer has a Scholars@Duke profile, so the information shown here reflects
their Duke status at the time this item was deposited.
Lori A. Setton
Adjunct Professor of Biomedical Engineering
Research in Setton's laboratory is focused on the role of mechanical factors in the
degeneration and repair of soft tissues of the musculoskeletal system, including the
intervertebral disc, articular cartilage and meniscus. Work in the Laboratory is focused
on engineering and evaluating materials for tissue regeneration and drug delivery.
Studies combining engineering and biology are also used to determine the role of mechanical
factors to promote and control healing of cartilaginous tissues. Re
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