Characterization of porous, dexamethasone-releasing polyurethane coatings for glucose sensors.

Loading...
Thumbnail Image

Date

2014-11

Authors

Klitzman, Bruce
Reichert, William M

Journal Title

Journal ISSN

Volume Title

Repository Usage Stats

252
views
382
downloads

Citation Stats

Abstract

Commercially available implantable needle-type glucose sensors for diabetes management are robust analytically but can be unreliable clinically primarily due to tissue-sensor interactions. Here, we present the physical, drug release and bioactivity characterization of tubular, porous dexamethasone (Dex)-releasing polyurethane coatings designed to attenuate local inflammation at the tissue-sensor interface. Porous polyurethane coatings were produced by the salt-leaching/gas-foaming method. Scanning electron microscopy and micro-computed tomography (micro-CT) showed controlled porosity and coating thickness. In vitro drug release from coatings monitored over 2 weeks presented an initial fast release followed by a slower release. Total release from coatings was highly dependent on initial drug loading amount. Functional in vitro testing of glucose sensors deployed with porous coatings against glucose standards demonstrated that highly porous coatings minimally affected signal strength and response rate. Bioactivity of the released drug was determined by monitoring Dex-mediated, dose-dependent apoptosis of human peripheral blood derived monocytes in culture. Acute animal studies were used to determine the appropriate Dex payload for the implanted porous coatings. Pilot short-term animal studies showed that Dex released from porous coatings implanted in rat subcutis attenuated the initial inflammatory response to sensor implantation. These results suggest that deploying sensors with the porous, Dex-releasing coatings is a promising strategy to improve glucose sensor performance.

Department

Description

Provenance

Citation

Published Version (Please cite this version)

10.1016/j.actbio.2014.07.019

Publication Info

Vallejo-Heligon, Suzana G, Bruce Klitzman and William M Reichert (2014). Characterization of porous, dexamethasone-releasing polyurethane coatings for glucose sensors. Acta Biomaterialia, 10(11). pp. 4629–4638. 10.1016/j.actbio.2014.07.019 Retrieved from https://hdl.handle.net/10161/9194.

This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.


Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.