A wirelessly controlled implantable LED system for deep brain optogenetic stimulation.

dc.contributor.author

Rossi, Mark A

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Go, Vinson

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Murphy, Tracy

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Fu, Quanhai

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Morizio, James

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Yin, Henry H

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Switzerland

dc.date.accessioned

2017-01-05T15:43:51Z

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2015

dc.description.abstract

In recent years optogenetics has rapidly become an essential technique in neuroscience. Its temporal and spatial specificity, combined with efficacy in manipulating neuronal activity, are especially useful in studying the behavior of awake behaving animals. Conventional optogenetics, however, requires the use of lasers and optic fibers, which can place considerable restrictions on behavior. Here we combined a wirelessly controlled interface and small implantable light-emitting diode (LED) that allows flexible and precise placement of light source to illuminate any brain area. We tested this wireless LED system in vivo, in transgenic mice expressing channelrhodopsin-2 in striatonigral neurons expressing D1-like dopamine receptors. In all mice tested, we were able to elicit movements reliably. The frequency of twitches induced by high power stimulation is proportional to the frequency of stimulation. At lower power, contraversive turning was observed. Moreover, the implanted LED remains effective over 50 days after surgery, demonstrating the long-term stability of the light source. Our results show that the wireless LED system can be used to manipulate neural activity chronically in behaving mice without impeding natural movements.

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/25713516

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https://hdl.handle.net/10161/13450

dc.language

eng

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Frontiers Media SA

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Front Integr Neurosci

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10.3389/fnint.2015.00008

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channelrhodopsin

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direct pathway

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freely-behaving

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optogenetics

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striatonigral

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wireless

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A wirelessly controlled implantable LED system for deep brain optogenetic stimulation.

dc.type

Journal article

duke.contributor.orcid

Morizio, James|0000-0002-1463-9257

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/25713516

pubs.begin-page

8

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Basic Science Departments

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Duke

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Duke Institute for Brain Sciences

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Electrical and Computer Engineering

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Institutes and Provost's Academic Units

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Neurobiology

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Pratt School of Engineering

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Psychology and Neuroscience

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School of Medicine

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Trinity College of Arts & Sciences

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University Institutes and Centers

pubs.publication-status

Published online

pubs.volume

9

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