Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults

Mesquita, Paulo HC

Lamb, Donald A

Godwin, Joshua S

Osburn, Shelby C

Ruple, Bradley A

Moore, Johnathon H

Vann, Christopher G

Huggins, Kevin W

Fruge, Andrew D

Young, Kaelin C

Kavazis, Andreas N

Roberts, Michael D

2024-01-11T17:30:18Z

2024-01-11T17:30:18Z

<jats:p>The aim of this study was to investigate the effects of resistance training (RT) on the redox status of skeletal muscle in older adults. Thirteen males aged 64 ± 9 years performed full-body RT 2x/week for 6 weeks. Muscle biopsies were obtained from the vastus lateralis prior to and following RT. The mRNA, protein, and enzymatic activity levels of various endogenous antioxidants were determined. In addition, skeletal muscle 4-hydroxynonenal and protein carbonyls were determined as markers of oxidative damage. Protein levels of heat shock proteins (HSPs) were also quantified. RT increased mRNA levels of all assayed antioxidant genes, albeit protein levels either did not change or decreased. RT increased total antioxidant capacity, catalase, and glutathione reductase activities, and decreased glutathione peroxidase activity. Lipid peroxidation also decreased and HSP60 protein increased following RT. In summary, 6 weeks of RT decreased oxidative damage and increased antioxidant enzyme activities. Our results suggest the older adult responses to RT involve multi-level (transcriptional, post-transcriptional, and post-translational) control of the redox status of skeletal muscle.</jats:p>

2076-3921

https://hdl.handle.net/10161/29760

en

MDPI AG

Antioxidants

10.3390/antiox10030350

https://creativecommons.org/licenses/by-nc/4.0

Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults

Journal article

350

350

3

Duke

School of Medicine

Staff

Institutes and Centers

Center for the Study of Aging and Human Development

Published online

10