Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.

Schulze, DH

Kelsoe, G

United States

2015-11-18T16:31:51Z

1987-07-01

The filter paper disc method for cloning inducible lymphocytes was used to census the splenic B cell population of C57BL/6 and BALB/c mice for the expression of three VH gene-families, VH X-24, -Q52, and -J558. B cell colonies, arising from single founder lymphocytes, were identified by in situ hybridization with VH family- and C mu-specific cDNA probes. Some 6.7 X 10(4) C mu+ colonies were screened. Among C57BL/6- or BALB/c-derived colonies, approximately 3% were VH X-24+, approximately 19% were VH Q52+, and approximately 54% were VH J558+. These frequencies are consistent with a process of equiprobable expression for individual VH segments, and provide direct evidence that normal splenic B lymphocytes use a process of random genetic combinatorics to generate the antibody repertoire.

http://www.ncbi.nlm.nih.gov/pubmed/3110348

0022-1007

https://hdl.handle.net/10161/10896

eng

Rockefeller University Press

J Exp Med

Animals

Antibody Diversity

B-Lymphocytes

Cell Division

Cell Line

DNA

Genetic Variation

Genotype

Immunoglobulin Heavy Chains

Immunoglobulin Variable Region

Lipopolysaccharides

Mice

Mice, Inbred BALB C

Mice, Inbred C57BL

Nucleic Acid Hybridization

Spleen

Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.

Journal article

http://www.ncbi.nlm.nih.gov/pubmed/3110348

163

172

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Basic Science Departments

Duke

Duke Cancer Institute

Duke Human Vaccine Institute

Immunology

Institutes and Centers

School of Medicine

Published

166