Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration.
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Cell delivery to the pathological intervertebral disc (IVD) has significant therapeutic potential for enhancing IVD regeneration. The development of injectable biomaterials that retain delivered cells, promote cell survival, and maintain or promote an NP cell phenotype in vivo remains a significant challenge. Previous studies have demonstrated NP cell - laminin interactions in the nucleus pulposus (NP) region of the IVD that promote cell attachment and biosynthesis. These findings suggest that incorporating laminin ligands into carriers for cell delivery may be beneficial for promoting NP cell survival and phenotype. Here, an injectable, laminin-111 functionalized poly(ethylene glycol) (PEG-LM111) hydrogel was developed as a biomaterial carrier for cell delivery to the IVD. We evaluated the mechanical properties of the PEG-LM111 hydrogel, and its ability to retain delivered cells in the IVD space. Gelation occurred in approximately 20 min without an initiator, with dynamic shear moduli in the range of 0.9-1.4 kPa. Primary NP cell retention in cultured IVD explants was significantly higher over 14 days when cells were delivered within a PEG-LM111 carrier, as compared to cells in liquid suspension. Together, these results suggest this injectable laminin-functionalized biomaterial may be an easy to use carrier for delivering cells to the IVD.
Published Version (Please cite this version)
Francisco, Aubrey T, Robert J Mancino, Robby D Bowles, Jonathan M Brunger, David M Tainter, Yi-Te Chen, William J Richardson, Farshid Guilak, et al. (2013). Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration. Biomaterials, 34(30). pp. 7381–7388. 10.1016/j.biomaterials.2013.06.038 Retrieved from https://hdl.handle.net/10161/7858.
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Research in Setton's laboratory is focused on the role of mechanical factors in the degeneration and repair of soft tissues of the musculoskeletal system, including the intervertebral disc, articular cartilage and meniscus. Work in the Laboratory is focused on engineering and evaluating materials for tissue regeneration and drug delivery. Studies combining engineering and biology are also used to determine the role of mechanical factors to promote and control healing of cartilaginous tissues. Research in the Laboratory is funded by The National Institutes of Health, The Coulter Foundation and The North Carolina Biotechnology Center.
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