Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice.

Tkatchenko, Andrei V

Luo, Xiaoyan

Tkatchenko, Tatiana V

Vaz, Candida

Tanavde, Vivek M

Maurer-Stroh, Sebastian

Zauscher, Stefan

Gonzalez, Pedro

Young, Terri L

Rota, Rossella

United States

2017-08-18T20:12:41Z

2017-08-18T20:12:41Z

2016

Development of myopia is associated with large-scale changes in ocular tissue gene expression. Although differential expression of coding genes underlying development of myopia has been a subject of intense investigation, the role of non-coding genes such as microRNAs in the development of myopia is largely unknown. In this study, we explored myopia-associated miRNA expression profiles in the retina and sclera of C57Bl/6J mice with experimentally induced myopia using microarray technology. We found a total of 53 differentially expressed miRNAs in the retina and no differences in miRNA expression in the sclera of C57BL/6J mice after 10 days of visual form deprivation, which induced -6.93 ± 2.44 D (p < 0.000001, n = 12) of myopia. We also identified their putative mRNA targets among mRNAs found to be differentially expressed in myopic retina and potential signaling pathways involved in the development of form-deprivation myopia using miRNA-mRNA interaction network analysis. Analysis of myopia-associated signaling pathways revealed that myopic response to visual form deprivation in the retina is regulated by a small number of highly integrated signaling pathways. Our findings highlighted that changes in microRNA expression are involved in the regulation of refractive eye development and predicted how they may be involved in the development of myopia by regulating retinal gene expression.

https://www.ncbi.nlm.nih.gov/pubmed/27622715

PONE-D-16-12243

1932-6203

https://hdl.handle.net/10161/15319

eng

Public Library of Science (PLoS)

PLoS One

10.1371/journal.pone.0162541

Animals

Disease Models, Animal

Gene Expression Profiling

Gene Regulatory Networks

Mice

Mice, Inbred C57BL

MicroRNAs

Myopia

Photic Stimulation

RNA, Messenger

Retina

Sclera

Sensory Deprivation

Signal Transduction

Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice.

Journal article

Zauscher, Stefan|0000-0002-2290-7178

Young, Terri L|0000-0001-6994-9941

https://www.ncbi.nlm.nih.gov/pubmed/27622715

e0162541

9

Chemistry

Clinical Science Departments

Duke

Mechanical Engineering and Materials Science

Ophthalmology

Pathology

Pratt School of Engineering

School of Medicine

Trinity College of Arts & Sciences

Published online

11