Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice.
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2016
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Development of myopia is associated with large-scale changes in ocular tissue gene expression. Although differential expression of coding genes underlying development of myopia has been a subject of intense investigation, the role of non-coding genes such as microRNAs in the development of myopia is largely unknown. In this study, we explored myopia-associated miRNA expression profiles in the retina and sclera of C57Bl/6J mice with experimentally induced myopia using microarray technology. We found a total of 53 differentially expressed miRNAs in the retina and no differences in miRNA expression in the sclera of C57BL/6J mice after 10 days of visual form deprivation, which induced -6.93 ± 2.44 D (p < 0.000001, n = 12) of myopia. We also identified their putative mRNA targets among mRNAs found to be differentially expressed in myopic retina and potential signaling pathways involved in the development of form-deprivation myopia using miRNA-mRNA interaction network analysis. Analysis of myopia-associated signaling pathways revealed that myopic response to visual form deprivation in the retina is regulated by a small number of highly integrated signaling pathways. Our findings highlighted that changes in microRNA expression are involved in the regulation of refractive eye development and predicted how they may be involved in the development of myopia by regulating retinal gene expression.
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Tkatchenko, Andrei V, Xiaoyan Luo, Tatiana V Tkatchenko, Candida Vaz, Vivek M Tanavde, Sebastian Maurer-Stroh, Stefan Zauscher, Pedro Gonzalez, et al. (2016). Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice. PLoS One, 11(9). p. e0162541. 10.1371/journal.pone.0162541 Retrieved from https://hdl.handle.net/10161/15319.
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Scholars@Duke
Stefan Zauscher
My research lies at the intersection of surface and colloid science, polymer materials engineering, and biointerface science, with four central areas of focus:
- Fabrication, manipulation and characterization of stimulus-responsive biomolecular and bio-inspired polymeric nanostructures on surfaces;
- Nanotechnology of soft-wet materials and hybrid biological/non-biological microdevices;
- Receptor-ligand interactions relevant to the diagnostics of infectious diseases;
- Friction of soft-wet materials, specifically the role of glycoproteins on friction in diarthroidal joints.
These four broad lines of inquiry deal with fundamental behaviors of soft-wet materials on surfaces and interfaces. The design and fabrication of these interfaces using "smart" polymeric and biomolecular nanostructures, and the characterization of the resulting structures, are critically important for the development of biomolecular sensors and devices and for bioinspired materials. Key approaches and tools I use in my research are: bottom-up organization on the molecular scale, through self-assembly, in-situ polymerization, and manipulation of intermolecular interactions; topdown fabrication, through scanning probe nanolithography; stimulus-responsive polymers; molecular recognition; and new approaches to sensing and manipulation. This research supports Duke's Pratt School of Engineering strategic initiative to expand research in soft-wet Materials Science.
Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.