Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.

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2009-01

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Abstract

We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing forces generated by applying a coverslip most likely distort some of the spherical liposomes into a tubular shape, and causes some to leak and incorporate FtsZ inside. We describe protocols for liposome preparation, and for preparing membrane-targeted FtsZ that can assemble contractile Z rings inside the tubular liposomes. We also describe the characterization of the multilamellar liposomes in terms of the permeability or leakiness for a small fluorescent dye and larger protein molecules. These liposomes may be useful for reconstitution of other biological systems.

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10.1016/s0076-6879(09)64001-5

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Osawa, Masaki, and Harold P Erickson (2009). Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings. Methods in enzymology, 464(C). pp. 3–17. 10.1016/s0076-6879(09)64001-5 Retrieved from https://hdl.handle.net/10161/22301.

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Scholars@Duke

Erickson

Harold Paul Erickson

James B. Duke Distinguished Professor Emeritus

Recent research has been on cytoskeleton (eukaryotes and bacteria); a skirmish to debunk the irisin story; a reinterpretation of proposed multivalent binders of the coronavirus spike protein. I have also published an ebook on "Principles of Protein-Protein Association" suitable for a course module or individual learning.


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