N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.


N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.





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Publication Info

Guan, Ziqiang, Antonia Delago, Phillip Nußbaum, Benjamin Meyer, Sonja-Verena Albers and Jerry Eichler (2016). N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier. FEBS Lett, 590(18). pp. 3168–3178. 10.1002/1873-3468.12341 Retrieved from https://hdl.handle.net/10161/13030.

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Ziqiang Guan

Research Professor in Biochemistry

We develop and apply mass spectrometry techniques to address biochemical and biomedical questions that are lipid-related. Research projects include:

1) Structural lipidomics

o   Develop and apply high resolution tandem mass spectrometry-based lipidomics for the discovery, structural elucidation and functional study of novel lipids.

2) Elucidation of novel pathways/enzymes of lipid biosynthesis and metabolism

o   Genetic, biochemical and MS approaches are employed to identify the substrates and pathways involved in lipid biosynthesis and metabolism

3) Identification of lipid biomarkers of genetic diseases and cancers

o    Provide molecular insights into the disease mechanisms, as well as to serve as the diagnostic and prognostic tools of diseases.

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